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首页> 外文期刊>Molecular and Cellular Biology >A nuclear gene of Saccharomyces cerevisiae needed for stable maintenance of plasmids.
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A nuclear gene of Saccharomyces cerevisiae needed for stable maintenance of plasmids.

机译：酿酒酵母的核基因对于稳定维持质粒是必需的。

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We have isolated host mutants of Saccharomyces cerevisiae in which the 2 microns plasmid is poorly maintained. All the mutants tested constituted one complementation group, which was designated map1 (maintenance of plasmid). Minichromosomes carrying a chromosomal replication origin and a centromere were affected in the mutants. Two types of hybrid plasmids generated in vivo and in vitro appeared to compensate for the mutations and had DNA regions containing multiple ARS (autonomously replicating sequence) or a set of 2 microns inverted repeat sequences. These results suggested that poor maintenance of plasmids was due to low levels of replication, probably at the initiation of replication.
机译：我们已分离出酿酒酵母的宿主突变体，其中2微米质粒的维护较差。所有测试的突变体组成一个互补组，称为map1（质粒的维护）。携带染色体复制起点和着丝粒的微型染色体在突变体中受到影响。体内和体外产生的两种类型的杂种质粒似乎可以补偿突变，并具有包含多个ARS（自主复制序列）或一组2微米反向重复序列的DNA区域。这些结果表明，质粒的不良维持是由于低水平的复制，可能是在复制开始时。

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