Transient expression of the beta interferon promoter in human cells.

S Xanthoudakis; D Alper; J Hiscott

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Transient expression of the beta interferon promoter in human cells.

机译：β干扰素启动子在人类细胞中的瞬时表达。

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A human transient expression assay was used to examine the inducible transcriptional activation of beta interferon (IFN-beta) and IFN-alpha 1 promoters in a homologous cellular environment. Use of 293 cells, an adenovirus DNA-transformed human embryonic kidney cell line, permitted Sendai virus-inducible expression of IFN-beta-CAT hybrid gene. Introduction of the simian virus 40 (SV40) enhancer 5' or 3' to the IFN-CAT gene increased basal (uninduced) levels of chloramphenicol acetyltransferase (CAT) activity; in one construct the SV40 enhancer--IFN-beta regulatory region combination increased the induced CAT activity 50- to 100-fold, suggesting that this may be a generally useful inducible enhancer-promoter combination. No expression from the IFN-alpha-CAT hybrid gene was detected in 293 cells, indicating that human epithelioid cells lack a factor required for expression of the IFN-alpha promoter. However, when the IFN-alpha regulatory region was combined with the SV40 enhancer, a low level of inducible CAT activity was detected in the human transient system.

机译：使用人类瞬时表达测定法来检查同源细胞环境中β干扰素（IFN-beta）和IFN-alpha 1启动子的诱导型转录激活。使用293细胞（一种腺病毒DNA转化的人类胚胎肾细胞系），可以使仙台病毒诱导表达IFN-β-CAT杂种基因。将猿猴病毒40（SV40）增强子5'或3'引入IFN-CAT基因可提高氯霉素乙酰基转移酶（CAT）活性的基础水平（未诱导）;在一个构建体中，SV40增强子-IFN-β调节区组合将诱导的CAT活性提高了50到100倍，表明这可能是一种普遍有用的诱导型增强子-启动子组合。在293细胞中未检测到来自IFN-α-CAT杂合基因的表达，表明人上皮细胞缺乏表达IFN-α启动子所需的因子。但是，当IFN-α调节区与SV40增强剂结合使用时，在人类瞬时系统中检测到了低水平的诱导CAT活性。

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《Molecular and Cellular Biology》 |1987年第10期|共6页
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S Xanthoudakis; D Alper; J Hiscott;
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中图分类细胞生物学;
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Transient expression of the beta interferon promoter in human cells.

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