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首页> 外文期刊>Molecular and Cellular Biology >The GCR1 gene encodes a positive transcriptional regulator of the enolase and glyceraldehyde-3-phosphate dehydrogenase gene families in Saccharomyces cerevisiae.
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The GCR1 gene encodes a positive transcriptional regulator of the enolase and glyceraldehyde-3-phosphate dehydrogenase gene families in Saccharomyces cerevisiae.

机译：GCR1基因编码酿酒酵母中的烯醇酶和3-磷酸甘油醛脱氢酶基因家族的正转录调节子。

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摘要

The intracellular concentrations of the polypeptides encoded by the two enolase (ENO1 and ENO2) and three glyceraldehyde-3-phosphate dehydrogenase (TDH1, TDH2, and TDH3) genes were coordinately reduced more than 20-fold in a Saccharomyces cerevisiae strain carrying the gcr1-1 mutation. The steady-state concentration of glyceraldehyde-3-phosphate dehydrogenase mRNA was shown to be approximately 50-fold reduced in the mutant strain. Overexpression of enolase and glyceraldehyde-3-phosphate dehydrogenase in strains carrying multiple copies of either ENO1 or TDH3 was reduced more than 50-fold in strains carrying the gcr1-1 mutation. These results demonstrated that the GCR1 gene encodes a trans-acting factor which is required for efficient and coordinate expression of these glycolytic gene families. The GCR1 gene and the gcr1-1 mutant allele were cloned and sequenced. GCR1 encodes a predicted 844-amino-acid polypeptide; the gcr1-1 allele contains a 1-base-pair insertion mutation at codon 304. A null mutant carrying a deletion of 90% of the GCR1 coding sequence and a URA3 gene insertion was constructed by gene replacement. The phenotype of a strain carrying this null mutation was identical to that of the gcr1-1 mutant strain.
机译：在带有gcr1- 1个突变。在突变菌株中，甘油醛-3-磷酸脱氢酶mRNA的稳态浓度显示降低了约50倍。在携带gcr1-1突变的菌株中，携带多个ENO1或TDH3拷贝的菌株中烯醇酶和3-磷酸甘油醛脱氢酶的过表达减少了50倍以上。这些结果证明，GCR1基因编码反式作用因子，其是有效和协调这些糖酵解基因家族表达所必需的。克隆并测序了GCR1基因和gcr1-1突变等位基因。 GCR1编码一个预测的844个氨基酸的多肽; gcr1-1等位基因在304位密码子处包含一个1个碱基对的插入突变。通过基因替换构建了一个缺失90％GCR1编码序列和URA3基因插入的无效突变体。携带该无效突变的菌株的表型与gcr1-1突变菌株的表型相同。

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