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首页> 外文期刊>Molecular and Cellular Biology >Positive and negative transcriptional control by heme of genes encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in Saccharomyces cerevisiae.
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Positive and negative transcriptional control by heme of genes encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in Saccharomyces cerevisiae.

机译：酿酒酵母中编码3-羟基-3-甲基戊二酰辅酶A还原酶的基因的血红素正向和负向转录控制。

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摘要

Responses of the yeast genes encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, HMG1 and HMG2, to in vivo changes in heme concentrations were investigated. Expression of the genes was determined by direct measurement of the mRNA transcribed from each gene, by direct assay of the enzyme activity encoded by each gene, and by measurement of the expression of lacZ fusions to the control regions of each gene. These studies indicated that expression of HMG1 was stimulated by heme, whereas expression of HMG2 was repressed by heme. The effect of heme on HMG1 expression was mediated by the HAP1 transcriptional regulator and was independent of HAP2. Thus, the genes encoding the 3-hydroxy-3-methylglutaryl coenzyme A reductase isozymes join a growing list of gene pairs that are regulated by heme in opposite ways.
机译：研究了编码3-羟基-3-甲基戊二酰辅酶A还原酶HMG1和HMG2的酵母基因对体内血红素浓度变化的响应。通过直接测量从每个基因转录的mRNA，通过直接测定每个基因编码的酶活性以及通过测量与每个基因的控制区的lacZ融合体的表达来确定基因的表达。这些研究表明HMG1的表达被血红素刺激，而HMG2的表达被血红素抑制。血红素对HMG1表达的影响是由HAP1转录调节因子介导的，并且独立于HAP2。因此，编码3-羟基-3-甲基戊二酰辅酶A还原酶同工酶的基因加入了越来越多的由血红素以相反方式调节的基因对。

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