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首页> 外文期刊>Molecular and Cellular Biology >Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone.
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Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone.

机译：啤酒酵母SST2基因的肺调节和序列：对信息素脱敏的模型。

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Strains of both haploid mating types containing sst2 mutations are altered in response to pheromone; MATa sst2 cells are supersensitive to alpha-factor, and MAT alpha sst2 cells are supersensitive to a-factor. This phenotype suggests that SST2 encodes a component of the pheromone response pathway that is common to both mating types. We have cloned the SST2 gene by isolation of multicopy plasmids that complement the sst2-1 mutation. One such plasmid contained a 4.5-kilobase HindIII fragment that was able to complement the sst2-1 mutation in high or low copy number, integrated at the SST2 locus, and resulted in an sst2 phenotype when disrupted, indicating that this fragment contained the SST2 gene. We identified the functional region of the complementing DNA fragment by transposon mutagenesis. Sequencing of this fragment identified an open reading frame encoding 698 amino acids at a position that correlated well with the functional region. Expression of an Sst2-beta-galactosidase fusion was haploid specific and induced by exposure to pheromone. We discuss a model in which induction of the SST2 product results in inhibition of a component of the pheromone response pathway, resulting in desensitization to pheromone.
机译：两种含有sst2突变的单倍体交配类型的菌株都响应信息素而发生改变。 MATa sst2细胞对α因子超敏感，而MAT alpha sst2细胞对a因子超敏感。该表型表明，SST2编码两种交配类型共有的信息素应答途径的一个成分。我们已经通过分离补充sst2-1突变的多拷贝质粒克隆了SST2基因。一个这样的质粒包含一个4.5碱基对的HindIII片段，该片段能够以高或低拷贝数补充sst2-1突变，整合在SST2基因座上，并在被破坏时产生sst2表型，表明该片段包含SST2基因。我们通过转座子诱变鉴定了互补DNA片段的功能区域。该片段的测序鉴定了在与功能区很好相关的位置上编码698个氨基酸的开放阅读框。 Sst2-β-半乳糖苷酶融合体的表达是单倍体特异性的，并且通过暴露于信息素而诱导。我们讨论了一个模型，其中SST2产物的诱导导致抑制信息素反应途径的成分，从而导致对信息素的脱敏。

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