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首页> 外文期刊>Molecular and Cellular Biology >Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae.
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Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae.

机译：酵母酿酒酵母交配过程中细胞融合所需基因的鉴定和调控。

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We have devised a screen for genes from the yeast Saccharomyces cerevisiae whose expression is affected by cell type or by the mating pheromones. From this screen we identified a gene, FUS1, whose pattern of expression revealed interesting regulatory strategies and whose product was required for efficient cell fusion during mating. Transcription of FUS1 occurred only in a and alpha cells, not in a/alpha cells, where it was repressed by a1 X alpha 2, a regulatory activity present uniquely in a/alpha cells. Transcription of FUS1 showed an absolute requirement for the products of five STE genes, STE4, STE5, STE7, STE11, and STE12. Since the activators STE4, STE5, and STE12 are themselves repressed by a1 X alpha 2, the failure to express FUS1 in a/alpha cells is probably the result of a cascade of regulatory activities; repression of the activators by a1 X alpha 2 in turn precludes transcription of FUS1. In addition to regulation of FUS1 by cell type, transcription from the locus increased 10-fold or more when a or alpha cells were exposed to the opposing mating pheromone. To investigate the function of the Fus1 protein, we created fus1 null mutants. In fus1 X fus1 matings, the cells of a mating pair adhered tightly and appeared to form zygotes. However, the zygotes were abnormal. Within the conjugation bridge the contained a partition that prevented nuclear fusion and mixing of organelles. The predicted sequence of the Fus1 protein (deduced from the FUS1 DNA sequence) and subcellular fractionation studies with Fus1-beta-galactosidase hybrid proteins suggest that Fus1 is a membrane or secreted protein. Thus, Fus1 may be located at a position within the cell where it is poised to catalyze cell wall or plasma membrane fusion.
机译：我们设计了一种筛选酵母酵母基因的表达，其表达受细胞类型或交配信息素的影响。从这个屏幕上，我们确定了一个基因FUS1，其表达模式揭示了有趣的调控策略，并且其产物是交配过程中有效细胞融合所必需的。 FUS1的转录仅发生在a和alpha细胞中，而不发生在a / alpha细胞中，在那里被a1 X alpha 2抑制，这种调节活性在a / alpha细胞中唯一存在。 FUS1的转录显示出对五个STE基因，STE4，STE5，STE7，STE11和STE12的产物的绝对需求。由于激活因子STE4，STE5和STE12本身会被a1 X alpha 2抑制，因此在a / alpha细胞中表达FUS1的失败可能是一系列调控活动的结果。 a1 X alpha 2对激活子的抑制又会阻止FUS1的转录。除了通过细胞类型调节FUS1外，当a或alpha细胞暴露于相反的交配信息素时，基因座的转录增加10倍或更多。为了研究Fus1蛋白的功能，我们创建了fus1 null突变体。在fus1 X fus1交配中，一对交配细胞紧密粘附并似乎形成合子。但是，受精卵是异常的。在共轭桥内部，包含一个防止核融合和细胞器混合的隔板。 Fus1蛋白的预测序列（从FUS1 DNA序列推导）以及使用Fus1-β-半乳糖苷酶杂合蛋白进行的亚细胞分级研究表明，Fus1是膜蛋白或分泌蛋白。因此，Fus1可以位于细胞内准备催化细胞壁或质膜融合的位置。

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