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首页> 外文期刊>Molecular and Cellular Biology >The sarcomeric actin CArG-binding factor is indistinguishable from the c-fos serum response factor.
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The sarcomeric actin CArG-binding factor is indistinguishable from the c-fos serum response factor.

机译：肌节肌动蛋白CArG结合因子与c-fos血清反应因子没有区别。

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摘要

The c-fos serum response element (SRE) and a sarcomeric actin promoter element (CArG box) are similar in sequence and are recognized, respectively, by the serum response factor (SRF) and the CArG-binding factor (CBF). Although the transcriptional controls for the c-fos and sarcomeric actin genes are rather different, SRF and CBF have been found to be indistinguishable by all criteria tested. They exhibited similar chromatographic properties, sedimentation rates, and temperature stabilities. In mobility shift assays, the SRE competed more strongly than the actin CArG box for formation of either the SRF-SRE or the CBF-CArG complex. The symmetric inverted repeat of the left side of the Xenopus cytoskeletal actin SRE also competed, even more strongly, for each complex. The site-specific binding of each protein was inhibited both by orthophenanthroline, whose effects were reversed by zinc addition, and by treatment with potato acid phosphatase. Furthermore, immune serum raised against the c-fos SRF also recognized the actin CBF. We discuss how transcriptional control of these diverse genes might be obtained with a single similar factor.
机译：c-fos血清反应元件（SRE）和肌节肌动蛋白启动子元件（CArG box）的序列相似，分别被血清反应因子（SRF）和CArG结合因子（CBF）识别。尽管c-fos和sarcomeric肌动蛋白基因的转录控制差异很大，但已发现SRF和CBF在所有测试标准中均无法区分。它们表现出相似的色谱特性，沉降速率和温度稳定性。在迁移率变动分析中，SRE在形成SRF-SRE或CBF-CArG复合物方面比肌动蛋白CArG盒竞争更激烈。非洲爪蟾细胞骨架肌动蛋白SRE左侧的对称反向重复序列也竞争着每种复合物，甚至更为强烈。邻菲咯啉可抑制每种蛋白质的位点特异性结合，邻菲咯啉的作用可通过添加锌和马铃薯酸性磷酸酶处理而逆转。此外，针对c-fos SRF产生的免疫血清也识别肌动蛋白CBF。我们讨论如何通过单个相似因子获得这些多样化基因的转录控制。

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