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首页> 外文期刊>Molecular and Cellular Biology >Transcription of testicular angiotensin-converting enzyme (ACE) is initiated within the 12th intron of the somatic ACE gene.
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Transcription of testicular angiotensin-converting enzyme (ACE) is initiated within the 12th intron of the somatic ACE gene.

机译：睾丸血管紧张素转换酶（ACE）的转录在体细胞ACE基因的第12个内含子内启动。

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Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase that catalyzes the conversion of angiotensin I to the potent vasoconstrictor angiotensin II. By analyzing cDNA and genomic DNA, we have constructed a consensus sequence encoding the testis isozyme of mouse ACE. Testis ACE cDNA contains 2,435 base pairs and encodes a protein of 732 amino acids. The N-terminal 66 amino acids are unique to the testis isozyme, while the remaining 666 are identical to the carboxyl half of mouse somatic ACE. The overall conservation of amino acid sequence between the testis isozymes of the mouse, rabbit, and human is 78 to 84%. The conservation of amino acids for the N-terminal domain uniquely expressed within the testis is 63 to 67% between these species. Primer extension and RNase protection experiments show that RNA transcription of the testis ACE isozyme begins 16 or 17 bases upstream from the translation start site. A sequence element resembling a TATA box is found 25 bases 5' of the transcription start site. To create its unique isozyme of ACE, the testis begins mRNA transcription in the middle of the exonic-intronic structure of somatic ACE, within a sequence treated as an intron by somatic tissues. Testis ACE is not the result of alternative RNA splicing but seems due to the start of transcription at a unique site within the ACE gene.
机译：血管紧张素转化酶（ACE）是一种含锌的二肽基羧肽酶，可催化血管紧张素I转化为有效的血管收缩剂血管紧张素II。通过分析cDNA和基因组DNA，我们构建了编码小鼠ACE睾丸同工酶的共有序列。睾丸ACE cDNA包含2,435个碱基对，并编码732个氨基酸的蛋白质。 N末端的66个氨基酸是睾丸同工酶所特有的，而其余的666个与小鼠体细胞ACE的羧基一半相同。小鼠，兔子和人的睾丸同工酶之间的氨基酸序列总体保守性为78％至84％。这些物种之间，在睾丸内唯一表达的N末端结构域的氨基酸保守性为63％到67％。引物延伸和RNase保护实验表明，睾丸ACE同工酶的RNA转录起始于翻译起始位点上游16或17个碱基。在转录起始位点的25个碱基的5'处发现了类似于TATA盒的序列元件。为了创建其独特的ACE同工酶，睾丸在体细胞ACE的内含子序列中，在体细胞ACE的外显子-内含子结构的中间开始mRNA转录。睾丸ACE不是替代性RNA剪接的结果，而是由于ACE基因内唯一位点的转录开始所致。

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