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首页> 外文期刊>Molecular and Cellular Biology >Regulation of N-myc gene expression: use of an adenovirus vector to demonstrate posttranscriptional control.
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Regulation of N-myc gene expression: use of an adenovirus vector to demonstrate posttranscriptional control.

机译：N-myc基因表达的调节：使用腺病毒载体来证明转录后控制。

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We present evidence that differences in the levels of N-myc mRNA among different cell types are the result of posttranscriptional control. First, we noted that while steady-state mouse N-myc mRNA could be detected only in fetal mouse brain, it was transcribed at an equivalent rate in adult brain, liver, spleen, and placenta and in fetal brain. Similarly, the human N-myc gene was transcribed at an equivalent rate in HeLa cells, which do not accumulate this RNA in the cytoplasm, and cell lines G401 (a Wilms tumor-derived cell line) and SKNMc (established from a primitive neuroepithelioma), which do express N-myc RNA. As expected, the N-myc promoter functioned at equivalent rates, as demonstrated by the level of a reporter gene, when introduced into these cell types by using a recombinant adenovirus vector. The suggestion that posttranscriptional mechanisms control the level of this RNA was supported by the observation that sequences in the N-myc third exon specifically decreased the level of E1A mRNA when these sequences were placed downstream of the E1A promoter in a recombinant adenovirus. Finally, we further localized these sequences to a 600-bp fragment of the third exon by introducing various subclones of this sequence downstream of the E1A promoter in both viral and plasmid vectors.
机译：我们提供的证据表明，不同细胞类型之间N-myc mRNA的水平差异是转录后控制的结果。首先，我们注意到，虽然仅在胎儿小鼠脑中可以检测到稳态小鼠N-myc mRNA，但其在成年脑，肝，脾，胎盘和胎儿脑中的转录率相同。同样，人类N-myc基因在HeLa细胞中以相同的速度转录，而HeLa细胞在细胞质中不积累该RNA，而细胞系G401（Wilms肿瘤衍生的细胞系）和SKNMc（从原始神经上皮瘤建立） ，它确实表达N-myc RNA。如所预期的，当通过使用重组腺病毒载体引入这些细胞类型时，N-myc启动子以相等的速率发挥功能，如报道基因的水平所证明。转录后机制控制该RNA水平的建议得到了以下观察结果的支持：在重组腺病毒中，将这些序列置于E1A启动子的下游时，N-myc第三外显子中的序列会特异性降低E1A mRNA的水平。最后，我们通过在病毒和质粒载体中的E1A启动子下游引入该序列的各个亚克隆，将这些序列进一步定位于第三外显子的600 bp片段。

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