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首页> 外文期刊>Molecular and Cellular Biology >Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
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Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.

机译：Ro小胞质核糖核蛋白是La核糖核蛋白的一个亚类：来自未感染哺乳动物细胞的Ro和La小核糖核蛋白的进一步表征。

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摘要

Small ribonucleic acid (RNA)-protein complexes precipitated by anti-Ro and anti-La antibodies from lupus patients have been examined with emphasis on their RNA components. In both ribonucleoprotein (RNP) classes, the numbers of different RNA molecules and their sequences vary between mouse and human cells. The complex mixtures of La RNAs include two previously sequenced 4.5S RNAs from mouse cells and 5S ribosomal RNA-like molecules from both mouse and human cells. All Ro and La RNAs possess 5-triphosphates. Some La RNAs have internal modifications typical of transfer RNAs. The Ro RNPs are quite stable and are localized by immunofluorescence in the cell cytoplasm, whereas the majority of the La RNPs turn over rapidly and reside in the nucleus. Despite these differences, reconstitution experiments show that the Ro particles carry the La as well as the Ro determinant. Studies using a nuclear transcription system demonstrate that most of the La RNAs are synthesized by RNA polymerase III. The possibility that the La protein(s) functions in the transcription or maturation of all RNA polymerase III transcripts is discussed.
机译：已经检查了狼疮患者的抗Ro和抗La抗体沉淀的小核糖核酸（RNA）蛋白复合物，重点是其RNA成分。在两种核糖核蛋白（RNP）类中，不同的RNA分子的数量及其序列在小鼠和人类细胞之间都不同。 La RNA的复杂混合物包括来自小鼠细胞的两个先前测序的4.5S RNA和来自小鼠和人类细胞的5S核糖体RNA样分子。所有的Ro和La RNA都具有5-三磷酸。一些La RNA具有典型的转移RNA内部修饰。 Ro RNP非常稳定，并通过免疫荧光定位在细胞质中，而大多数La RNP迅速翻转并驻留在细胞核中。尽管存在这些差异，但重建实验表明，Ro粒子携带La以及Ro决定簇。使用核转录系统的研究表明，大多数La RNA是由RNA聚合酶III合成的。讨论了La蛋白在所有RNA聚合酶III转录本的转录或成熟中起作用的可能性。

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