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首页> 外文期刊>Molecular and Cellular Biology >In vitro translation of the intermediate filament proteins desmin and vimentin.
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In vitro translation of the intermediate filament proteins desmin and vimentin.

机译：中间丝蛋白desmin和波形蛋白的体外翻译。

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Polyadenylated ribonucleic acid (RNA) was isolated from chicken skeletal and smooth muscle and translated in a cell-free rabbit reticulocyte system. Both types of muscle tissue contain messenger RNAs that code for the intermediate filament proteins desmin and vimentin, and the relative concentrations of the two translation products reflect the prevalence of the two proteins in vivo. Desmin synthesis represents a greater proportion of the total protein synthesis from smooth muscle RNA than from skeletal muscle RNA, whereas the converse is true of vimentin synthesis. Fractionation of the RNA on formamide-containing sucrose gradients before translation indicates that the desmin messenger RNA is larger than the vimentin messenger RNA and contains an extensive noncoding segment. The desmin and vimentin messages code predominantly for the non-phosphorylated forms of desmin and vimentin. However, the ratio of phosphorylated to unphosphorylated forms of the proteins could be increased by adding cyclic adenosine monophosphate-dependent kinase activity to the translation mixtures. These results suggest that desmin and vimentin are each synthesized from a single messenger RNA species and that posttranslational phosphorylation generates the additional isoelectric variants of each which are observed in vivo.
机译：从鸡骨骼肌和平滑肌中分离出聚腺苷酸核糖核酸（RNA），并在无细胞的兔网织红细胞系统中进行翻译。两种类型的肌肉组织都包含编码中间丝蛋白desmin和波形蛋白的信使RNA，两种翻译产物的相对浓度反映了两种蛋白在体内的普遍性。结蛋白的合成在平滑肌RNA的总蛋白合成中所占的比例要比在骨骼肌RNA的合成中所占的比例更高，而波形蛋白的合成则相反。在翻译前将RNA在含甲酰胺的蔗糖梯度上进行分级分离，表明结蛋白信使RNA大于波形蛋白信使RNA，并包含一个广泛的非编码片段。结蛋白和波形蛋白的信息主要编码结蛋白和波形蛋白的非磷酸化形式。但是，可以通过向翻译混合物中添加环磷酸腺苷依赖性激酶活性来增加蛋白质的磷酸化形式与非磷酸化形式的比率。这些结果表明，结蛋白和波形蛋白均由单个信使RNA种类合成，并且翻译后磷酸化作用可产生在体内观察到的每种等离子变体。

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