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首页> 外文期刊>Molecular and Cellular Biology >Generation of a variant t(2;8) translocation of Burkitt's lymphoma by site-specific recombination via the kappa light-chain joining signals.
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Generation of a variant t(2;8) translocation of Burkitt's lymphoma by site-specific recombination via the kappa light-chain joining signals.

机译：通过kappa轻链连接信号通过位点特异性重组产生伯基特氏淋巴瘤的t（2; 8）变体易位。

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摘要

We analyzed the Burkitt's lymphoma line BL64 in which a reciprocal translocation joins the immunoglobulin kappa light-chain locus on chromosome 2 to the c-myc gene on chromosome 8. The breakpoints on the two marker chromosomes 8+ and 2p- occurred 5' of the Js segment within the conserved nonamer and heptamer recombination sequences. Both signals were detected directly adjacent to the breakpoints in sequences of chromosome 8 suggesting that the translocation in BL64 was catalyzed by enzymes normally involved in V-J recombination. The distance between the c-myc gene and the breakpoint in J kappa amounts to at least 90 kilobases on the DNA level. In one allele of the c-myc gene somatic mutations were found in the promoter-leader region. This allele is transcribed and is supposed to be involved in the translocation. The half-life of the c-myc-specific mRNA in BL64 cells is not prolonged in comparison to the normal c-myc message. These results suggest that in Burkitt's lymphoma the translocation occurs during an early stage of B-cell differentiation and that in the variant translocations mechanisms other than a prolonged half-life, such as changes in transcriptional rates, or other posttranscriptional RNA processing contribute to the high steady-state level of c-myc RNA in the cytoplasm.
机译：我们分析了伯基特氏淋巴瘤BL64系，其中一个相互易位将2号染色体上的免疫球蛋白轻链基因座连接到8号染色体上的c-myc基因。两个标记8+和2p-染色体的断裂点位于保守的九聚体和七聚体重组序列内的Js区段。直接在8号染色体序列的断点附近检测到这两个信号，表明BL64中的易位是由通常参与V-J重组的酶催化的。 c-myc基因与J kappa断裂点之间的距离至少为DNA水平的90千个碱基。在c-myc基因的一个等位基因中，在启动子-前导区发现了体细胞突变。该等位基因被转录，并被认为参与了易位。与正常c-myc信息相比，BL-64细胞中c-myc特异性mRNA的半衰期没有延长。这些结果表明，在伯基特氏淋巴瘤中，易位发生在B细胞分化的早期阶段，并且在变异型易位机制中，除了延长的半衰期（例如转录速率的变化或其他转录后RNA加工）以外，还导致了高细胞质中c-myc RNA的稳态水平。

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