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首页> 外文期刊>Molecular and Cellular Biology >Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro.
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Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro.

机译：非洲爪蟾的转录无活性卵母细胞型5S RNA基因与TFIIIA在体外复合。

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摘要

An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the whole oocyte extract, a promoter-binding protein required for 5S RNA gene transcription, TFIIIA, was bound both to the actively transcribed somatic-type 5S RNA gene and to the largely inactive oocyte-type 5S RNA genes. These findings suggest that the mechanism for the differential expression of 5S RNA genes during Xenopus development does not involve differential binding of TFIIIA to 5S RNA genes.
机译：爪蟾全卵母细胞的提取物显示出比卵母细胞型5S RNA基因转录体细胞5S RNA基因的效率高约100倍。该偏好比将5S RNA基因显微注射到卵母细胞核中或在卵母细胞核提取物中体外转录时所看到的偏好至少大10倍。在整个卵母细胞提取物中体外观察到的支持体细胞型5S RNA基因的大约100倍的转录偏向类似于在发育中的非洲爪蟾胚胎中观察到的转录偏向。我们还表明，在整个卵母细胞提取物中，5S RNA基因转录所需的启动子结合蛋白TFIIIA既与活跃转录的体细胞型5S RNA基因结合，又与基本上无活性的卵母细胞型5S RNA基因结合。这些发现表明非洲爪蟾发育过程中5S RNA基因差异表达的机制不涉及TFIIIA与5S RNA基因的差异结合。

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