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首页> 外文期刊>Molecular and Cellular Biology >Comparison of the structure and cell cycle expression of mRNAs encoded by two histone H3-H4 loci in Saccharomyces cerevisiae.
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Comparison of the structure and cell cycle expression of mRNAs encoded by two histone H3-H4 loci in Saccharomyces cerevisiae.

机译：酿酒酵母中两个组蛋白H3-H4基因座编码的mRNA的结构和细胞周期表达的比较。

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摘要

The haploid genome of Saccharomyces cerevisiae contains two nonallelic sets of histone H3 and H4 gene pairs, termed the copy I and copy II loci. The structures of the mRNA transcripts from each of these four genes were examined by nuclease protection and primer extension mapping. For each gene, several species of mRNAs were identified that differed in the lengths of their 5' and 3' untranslated regions. The cell cycle accumulation pattern of the H3 and H4 mRNAs was determined in cells from early-exponential-growth cultures fractionated by centrifugal elutriation. The RNA transcripts from all four genes were regulated with the cell division cycle, and transcripts from the nonallelic gene copies showed tight temporal coordination. Cell cycle regulation did not depend on selection of a particular histone mRNA transcript since the ratio of the multiple species from each gene remained the same across the division cycle. Quantitative measurements showed significant differences in the amounts of mRNA expressed from the two nonallelic gene sets. The mRNAs from the copy II H3 and H4 genes were five to seven times more abundant than the mRNAs from the copy I genes. There was no dosage compensation in the steady-state levels of mRNA when either set of genes was deleted. In particular, there was no increase in the amount of copy I H3 or H4 transcripts in cells in which the high-abundance copy II genes were deleted.
机译：酿酒酵母的单倍体基因组包含两个非等位基因组的组蛋白H3和H4基因对，称为复制I和复制II位点。通过核酸酶保护和引物延伸图谱检查了这四个基因中每个基因的mRNA转录物的结构。对于每个基因，鉴定出几种mRNA，它们的5'和3'非翻译区的长度不同。 H3和H4 mRNA的细胞周期积累模式确定了通过离心淘析分离的早期指数生长培养物中的细胞。来自所有四个基因的RNA转录物均受细胞分裂周期调控，来自非等位基因拷贝的转录物表现出紧密的时间协调性。细胞周期调控不取决于特定组蛋白mRNA转录物的选择，因为来自每个基因的多种物种的比率在整个分裂周期中保持相同。定量测量显示，从两个非等位基因组表达的mRNA数量存在显着差异。来自复制II H3和H4基因的mRNA比来自复制I基因的mRNA丰富五到七倍。当删除任一组基因时，mRNA的稳态水平没有剂量补偿。特别地，在其中删除了高丰度拷贝II基因的细胞中，拷贝I H3或H4转录本的数量没有增加。

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