Histones are positively charged proteins that play a critical role in epigenetic regulation. Histone Post Translational Modification (PTM) leads to alterations of transcription factor access to DNA as well as interactions with other affecter mechanisms ultimately resulting in changes in protein expression. These changes in protein expression have been associated with several disease states (Figure 1) hence study will provide valuable insights in the search for biomarkers and potential therapeutic targets. Using a novel method combining yeast genetics, stable isotope labeling of amino acids in cell culture (SILAC), mass spectrometry and bioinformatics analysis we will mutate and determine the proteomic effects that result on specific histone PTM sites.
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