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首页> 外文期刊>Molecular and Cellular Biology >Delineation of DNA sequences that are important for in vitro transcription from the adenovirus EIIa late promoter.
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Delineation of DNA sequences that are important for in vitro transcription from the adenovirus EIIa late promoter.

机译：描绘对于从腺病毒EIIa晚期启动子进行体外转录非常重要的DNA序列。

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摘要

Late in infection, transcription of the EIIa gene is initiated primarily at map unit 72 of the adenovirus genome. A cell-free nuclear extract system was used to determine sequence elements important for the function of this late promoter. In such a system, the transcriptional activity of a circular template was found to be much higher (5- to 10-fold) than that of a linear template. The effect of template topology appeared to be dependent on two distal upstream elements with 5' boundaries located near -265 to -223 and -147 to -133 (in relation to the initiation site), since deletions of these regions reduced transcription of the circular template, in a stepwise fashion, to a level similar to that observed with the linear template. Further deletions revealed an element in the -116 region that appeared to be more important for transcription of the circular template (10-fold reduction) than for transcription of the linear template (3-fold reduction). Lastly, deletion of the TACAAA sequence in the -29 region resulted in further reduction in transcription, indicating that this element functions as a promoter in vitro.
机译：感染后期，EIIa基因的转录主要在腺病毒基因组的图谱单元72开始。使用无细胞核提取物系统确定对该晚期启动子的功能重要的序列元件。在这种系统中，发现圆形模板的转录活性比线性模板的转录活性高得多（5至10倍）。模板拓扑的作用似乎取决于5'边界位于-265至-223和-147至-133（相对于起始位点）附近的两个远端上游元件，因为这些区域的缺失降低了环状分子的转录模板以逐步的方式，达到与线性模板所观察到的相似的水平。进一步的删除揭示了-116区域中的一个元素，对于环状模板的转录（减少10倍），似乎比线性模板的转录（减少3倍）更重要。最后，在-29区中TACAAA序列的缺失导致转录的进一步降低，表明该元件在体外起启动子的作用。

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