Transfection of a human gene that corrects the Lec1 glycosylation defect: evidence for transfer of the structural gene for N-acetylglucosaminyltransferase I.

R Kumar; P Stanley

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Transfection of a human gene that corrects the Lec1 glycosylation defect: evidence for transfer of the structural gene for N-acetylglucosaminyltransferase I.

机译：纠正Lec1糖基化缺陷的人类基因的转染：N-乙酰氨基葡萄糖氨基转移酶I结构基因转移的证据。

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摘要

Chinese hamster ovary (CHO) glycosylation mutants provide an approach to cloning mammalian glycosyltransferases by transfection and gene rescue. In this paper, complementation of the lec1 CHO mutation by human DNA is described. Lec1 transfectants expressed human N-acetylglucosaminyltransferase I (GlcNAc-TI) activity and possessed common human DNA fragments. Cloning of GlcNAc-TI should therefore be possible.

机译：中国仓鼠卵巢（CHO）糖基化突变体提供了一种通过转染和基因拯救来克隆哺乳动物糖基转移酶的方法。在本文中，描述了人类DNA对lec1 CHO突变的互补作用。 Lec1转染子表达人N-乙酰氨基葡萄糖氨基转移酶I（GlcNAc-TI）活性，并具有常见的人DNA片段。因此，应该可以克隆GlcNAc-TI。

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《Molecular and Cellular Biology》 |1989年第12期|共5页
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R Kumar; P Stanley;
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中图分类细胞生物学;
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1. Five Lec1 CHO cell mutants have distinct Mgat1 gene mutations that encode truncated N-acetylglucosaminyltransferase I. [J] . Chen W, Stanley P Glycobiology. . 2003,第1期

机译：五个Lec1 CHO细胞突变体具有不同的Mgat1基因突变，它们编码截短的N-乙酰氨基葡萄糖氨基转移酶I。
2. Engineering novel Lec1 glycosylation mutants in CHO-DUKX cells: Molecular insights and effector modulation of N-acetylglucosaminyltransferase I [J] . Zhong X., Cooley C., Seth N., Biotechnology and Bioengineering . 2012,第7期

机译：CHO-DUKX细胞中的工程新型Lec1糖基化突变体：N-乙酰氨基葡萄糖氨基转移酶I的分子洞察和效应子调节
3. Reduction of the Gal-α1,3-Gal Epitope of Mouse Endothelial Cells by Transfection with the N-Acetylglucosaminyltransferase III Gene [J] . Tae-Wook Chung, Kyoung-Sook Kim, Cheorl-Ho Kim Molecules and cells . 2003,第3期

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4. Gene expression inhibition of N-Myc downregulated gene 1 (NDRG1) monitoring and facilitation via transfectional transfer of NDRG1-siRNA constructs into- in vitro-cultured human glioblastoma cells [C] . Said Harun M., Polat Buelent, Guckenberger Mathias, 2011 4th International Conference on Biomedical Engineering and Informatics . 2011

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5. Identification of genes differentially expressed in the LNCaP human prostate cancer cell line following transfection with glutathione S-transferase P1. [D] . Cornblatt, Brian Scott. 2005

机译：谷胱甘肽S-转移酶P1转染后在LNCaP人前列腺癌细胞系中差异表达的基因的鉴定。
6. Transfection of a human gene that corrects the Lec1 glycosylation defect: evidence for transfer of the structural gene for N-acetylglucosaminyltransferase I. [O] . R Kumar, P Stanley 1989

机译：纠正Lec1糖基化缺陷的人类基因的转染：N-乙酰氨基葡萄糖氨基转移酶I结构基因转移的证据。
7. Transfection of a human gene that corrects the Lec1 glycosylation defect: evidence for transfer of the structural gene for N-acetylglucosaminyltransferase I. [O] . Kumar, R, Stanley, P 1989

机译：纠正Lec1糖基化缺陷的人类基因的转染：N-乙酰氨基葡萄糖氨基转移酶I结构基因转移的证据。
8. Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes. [R] . Kenny, J. J. 1992

机译：用重排免疫球蛋白基因转染小鼠和人造血祖细胞。

Transfection of a human gene that corrects the Lec1 glycosylation defect: evidence for transfer of the structural gene for N-acetylglucosaminyltransferase I.

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