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首页> 外文期刊>Molecular and Cellular Biology >Heteroduplex DNA correction in Saccharomyces cerevisiae is mismatch specific and requires functional PMS genes.
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Heteroduplex DNA correction in Saccharomyces cerevisiae is mismatch specific and requires functional PMS genes.

机译：酿酒酵母中的异源双链DNA校正是错配特异性的，需要功能性PMS基因。

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摘要

In vitro-constructed heteroduplex DNAs with defined mismatches were corrected in Saccharomyces cerevisiae cells with efficiencies that were dependent on the mismatch. Single-nucleotide loops were repaired very efficiently; the base/base mismatches G/T, A/C, G/G, A/G, G/A, A/A, T/T, T/C, and C/T were repaired with a high to intermediate efficiency. The mismatch C/C and a 38-nucleotide loop were corrected with low efficiency. This substrate specificity pattern resembles that found in Escherichia coli and Streptococcus pneumoniae, suggesting an evolutionary relationship of DNA mismatch repair in pro- and eucaryotes. Repair of the listed mismatches was severely impaired in the putative S. cerevisiae DNA mismatch repair mutants pms1 and pms2. Low-efficiency repair also characterized pms3 strains, except that correction of single-nucleotide loops occurred with an efficiency close to that of PMS wild-type strains. A close correlation was found between the repair efficiencies determined in this study and the observed postmeiotic segregation frequencies of alleles with known DNA sequence. This suggests an involvement of DNA mismatch repair in recombination and gene conversion in S. cerevisiae.
机译：具有确定的错配的体外构建的异源双链DNA在酿酒酵母细胞中得到了纠正，其效率取决于错配。单核苷酸环被非常有效地修复。基本/基本不匹配G / T，A / C，G / G，A / G，G / A，A / A，T / T，T / C和C / T以高至中等效率修复。错配C / C和38个核苷酸的环被低效率地纠正。这种底物特异性模式类似于在大肠杆菌和肺炎链球菌中发现的，表明原核生物和真核生物中DNA错配修复的进化关系。推定的酿酒酵母DNA失配修复突变体pms1和pms2严重损害了所列错配的修复。低效率修复也表征了pms3菌株，除了单核苷酸环的校正发生效率接近PMS野生型菌株。在这项研究中确定的修复效率与具有已知DNA序列的等位基因的减数分裂后分离频率之间发现了密切的相关性。这表明在酿酒酵母中DNA错配修复参与重组和基因转化。

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