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首页> 外文期刊>Molecular and Cellular Biology >E1A control of gene expression is mediated by sequences 5' to the transcriptional starts of the early viral genes.
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E1A control of gene expression is mediated by sequences 5' to the transcriptional starts of the early viral genes.

机译：E1A对基因表达的控制由早期病毒基因转录起始序列5'介导。

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摘要

A product of the adenovirus E1A gene is a positive regulator of early viral gene expression. In this report we show that E1A regulates at the transcriptional level and that sequences located 5' to the early viral regions contain sites which confer regulation by the E1A gene product. We constructed chimeric genes in which the sequences at the 5' end of the E2A, E3, and E4 regions were fused to the structural sequences of either the herpes simplex virus thymidine kinase gene, the bacterial gene encoding the enzyme neomycin phosphotransferase, or the chloramphenicol acetyltransferase gene. In all cases, expression of the chimeric genes was induced by a product of the E1A region. It was also found that the insertion of a fragment from the left-hand end of the adenovirus type 5 genome into a plasmid harboring the thymidine kinase gene resulted in elevated frequencies of transformation of TK- cells to TK+. The elevated transformation frequencies were only detected when the insert and tk gene were covalently joined. This effect occurred even when the insert was several kilobase upstream from, and regardless of its orientation to, the transcriptional initiation site of the tk gene. We propose that this region of the adenovirus type 5 genome harbors a cis-acting enhancer of transcription.
机译：腺病毒E1A基因的产物是早期病毒基因表达的正调节剂。在本报告中，我们显示了E1A在转录水平上调控，并且位于早期病毒区域5'的序列包含赋予E1A基因产物调控作用的位点。我们构建了嵌合基因，其中E2A，E3和E4区5'端的序列与单纯疱疹病毒胸苷激酶基因，编码酶新霉素磷酸转移酶或氯霉素的细菌基因的结构序列融合乙酰转移酶基因。在所有情况下，嵌合基因的表达都是由E1A区的产物诱导的。还发现将来自5型腺病毒基因组左手端的片段插入到具有胸苷激酶基因的质粒中导致TK-细胞向TK +转化的频率升高。仅当insert和tk基因共价结合时才检测到升高的转化频率。即使该插入片段位于tk基因转录起始位点上游几千碱基处，而无论其方向如何，也会发生这种作用。我们建议，腺病毒5型基因组的这一区域具有顺式作用转录增强子。

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