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首页> 外文期刊>Molecular and Cellular Biology >Activation of the cellular src gene by transducing retrovirus.
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Activation of the cellular src gene by transducing retrovirus.

机译：通过转导逆转录病毒激活细胞src基因。

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摘要

Newly isolated strains of avian sarcoma virus, S1 and S2, were shown to have the transduced cellular src gene as their viral transforming gene (Yamagishi et al., Virology 137:266-275, 1984). In this work, the S1 and S2 genomes were molecularly cloned, and the junction sequences between the viral genomes and the c-src genes and the complete nucleotide sequences of the v-src genes transduced in these viruses were determined. Data on the junction sequences suggested that 5' recombination had occurred between the 5'-noncoding region of c-src and the 5' region of the gag sequence encoding p19 in both viruses and that 3' recombination had occurred in the last coding exon of c-src with either the middle portion of the env sequence encoding gp85 for S1 or the 3' portion of pol coding for reverse transcriptase for S2. Comparison of the amino acid sequences of the S1 and S2 src products deduced from the nucleotide sequences (pp62S1-src and pp62S2-src with that of c-src protein (pp60c-src) indicated that in pp62S1-src the 8 carboxy-terminal amino acid residues of the total of 533 in pp60c-src are replaced by 43 residues translated from the env sequence at the wrong frame. In pp62S2-src, on the other hand, the 14 carboxy-terminal amino acids of pp60c-src are replaced by the 38 carboxy-terminal residues of reverse transcriptase. The mechanism of c-src transduction and the structural changes necessary for pp60c-src activation are discussed.
机译：已显示新分离的禽肉瘤病毒株S1和S2具有转导的细胞src基因作为其病毒转化基因（Yamagishi等人，Virology 137：266-275，1984）。在这项工作中，分子克隆了S1和S2基因组，并确定了病毒基因组与c-src基因之间的连接序列以及在这些病毒中转导的v-src基因的完整核苷酸序列。有关连接序列的数据表明，在两种病毒中，c-src的5'-非编码区与编码p19的gag序列的5'区域之间均发生了5'重组，而在该病毒的最后一个编码外显子中发生了3'重组。 c-src，env序列的中间部分编码S1的gp85或pol的3'部分编码S2的逆转录酶。从核苷酸序列（pp62S1-src和pp62S2-src与c-src蛋白（pp60c-src））推导的S1和S2 src产物的氨基酸序列比较表明，在pp62S1-src中有8个羧基末端的氨基pp60c-src中总共533个氨基酸残基被错误序列的env序列翻译而来的残基替换;而在pp62S2-src中，pp60c-src中的14个羧基末端氨基酸被替换为讨论了逆转录酶的38个羧基末端残基，探讨了c-src转导的机理以及激活pp60c-src所需的结构变化。

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