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首页> 外文期刊>Molecular and Cellular Biology >Regulation of synthesis and turnover of an interferon-inducible mRNA.
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Regulation of synthesis and turnover of an interferon-inducible mRNA.

机译：干扰素诱导mRNA的合成和转换的调节。

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摘要

Regulation of synthesis and turnover of an interferon (IFN)-inducible mRNA, mRNA 561, in HeLa monolayer cells was studied. Cytoplasmic levels of this mRNA were estimated by hybridization analyses with a cDNA clone that we have isolated as a probe. IFN-alpha A induced a high level of this mRNA in a transient fashion, whereas no induction was observed in response to IFN-gamma. Surprisingly little mRNA 561 was induced in cells treated simultaneously with IFN-alpha A and an inhibitor of protein synthesis, suggesting that in addition to IFN-alpha A, an interferon-inducible protein was needed for induction of this mRNA. Apparently this putative protein could be induced by IFN-gamma as well. Thus, although little mRNA 561 was synthesized in cells treated either with IFN-gamma alone or with IFN-alpha A and cycloheximide, a large quantity of this mRNA was induced in cells which had been pretreated with IFN-gamma and then treated with IFN-alpha A and cycloheximide. Once mRNA 561 was induced by IFN-alpha A, it turned over rapidly. This rapid turnover could be blocked by actinomycin D or cycloheximide indicating that another IFN-inducible protein may mediate this process.
机译：研究了HeLa单层细胞中干扰素（IFN）诱导的mRNA，mRNA 561的合成和更新的调控。通过与我们已分离出的cDNA克隆进行杂交分析，估算了该mRNA的细胞质水平。 IFN-αA以瞬时方式诱导了高水平的该mRNA，而未观察到对IFN-γ的诱导。令人惊讶的是，在同时用IFN-αA和蛋白质合成抑制剂处理的细胞中几乎没有诱导出mRNA 561，这表明除了IFN-αA之外，还需要干扰素诱导性蛋白来诱导该mRNA。显然，该推定蛋白也可以被IFN-γ诱导。因此，尽管在单独用IFN-γ或用IFN-αA和环己酰亚胺处理的细胞中几乎没有合成mRNA 561，但是在已经用IFN-γ预处理然后用IFN-γ处理的细胞中诱导了大量这种mRNA。 αA和环己酰亚胺。一旦IFN-αA诱导了mRNA 561，它就会迅速翻转。这种快速转换可能被放线菌素D或环己酰亚胺阻断，这表明另一种IFN诱导型蛋白可能介导了这一过程。

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