Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins.

P J Schatz; L Pillus; P Grisafi; F Solomon; D Botstein

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Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins.

机译：酵母酿酒酵母的两个功能性α-微管蛋白基因编码不同的蛋白质。

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Two alpha-tubulin genes from the budding yeast Saccharomyces cerevisiae were identified and cloned by cross-species DNA homology. Nucleotide sequencing studies revealed that the two genes, named TUB1 and TUB3, encoded gene products of 447 and 445 amino acids, respectively, that are highly homologous to alpha-tubulins from other species. Comparison of the sequences of the two genes revealed a 19% divergence between the nucleotide sequences and a 10% divergence between the amino acid sequences. Each gene had a single intervening sequence, located at an identical position in codon 9. Cell fractionation studies showed that both gene products were present in yeast microtubules. These two genes, along with the TUB2 beta-tubulin gene, probably encode the entire complement of tubulin in budding yeast cells.

机译：通过种间DNA同源性鉴定并克隆了来自萌芽的酿酒酵母中的两个α-微管蛋白基因。核苷酸测序研究表明，两个基因分别命名为TUB1和TUB3，分别编码447和445个氨基酸的基因产物，与其他物种的α-微管蛋白高度同源。比较两个基因的序列，发现核苷酸序列之间有19％的差异，氨基酸序列之间有10％的差异。每个基因都有一个插入序列，位于密码子9的相同位置。细胞分级研究表明，两种基因产物都存在于酵母微管中。这两个基因与TUB2β-微管蛋白基因一起，可能编码出芽酵母细胞中微管蛋白的整个补体。

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《Molecular and Cellular Biology》 |1986年第11期|共11页
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P J Schatz; L Pillus; P Grisafi; F Solomon; D Botstein;
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中图分类细胞生物学;
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1. Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins. [J] . P J Schatz, L Pillus, P Grisafi, Molecular and Cellular Biology . 1986,第11期

机译：酵母酿酒酵母的两个功能性α-微管蛋白基因编码不同的蛋白质。
2. Two divergent MET10 genes, one from Saccharomyces cerevisiae and one from Saccharomyces carlsbergensis, encode the alpha subunit of sulfite reductase and specify potential binding sites for FAD and NADPH. [J] . J Hansen, H Cherest, M C Kielland-Brandt Journal of bacteriology . 1994,第19期

机译：两个不同的MET10基因，一个来自酿酒酵母，一个来自酿酒酵母，编码亚硫酸盐还原酶的α亚基，并指定了FAD和NADPH的潜在结合位点。
3. Isolation and characterization of conditional-lethal mutations in the TUB1 alpha-tubulin gene of the yeast Saccharomyces cerevisiae. [J] . D Botstein, F Solomon, P J Schatz Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1988,第3期

机译：酵母酿酒酵母的TUB1α-微管蛋白基因中条件致死突变的分离和鉴定。
4. Experimental design trade-offs for gene regulatory network inference: An in silico study of the yeast Saccharomyces cerevisiae cell cycle [C] . Johan Markdahl, Nicolo Colombo, Johan Thunberg, IEEE Annual Conference on Decision and Control . 2017

机译：基因调节网络推论的实验设计折衷：酵母啤酒酵母细胞周期的计算机研究
5. Construction of lactose-fermenting and high-ethanol-producing hybrid yeast by protoplast fusion of Saccharomyces cerevisiae and Kluyveromyces fragilis and the analysis of beta-galactosidase gene in the hybrid yeast. [D] . Farahnak, Fereydoon. 1988

机译：通过酿酒酵母和脆弱克鲁维酵母的原生质体融合构建乳糖发酵和高产乙醇的杂交酵母，并分析该杂交酵母中的β-半乳糖苷酶基因。
6. Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins. [O] . P J Schatz, L Pillus, P Grisafi, 1986

机译：酵母酿酒酵母的两个功能性α-微管蛋白基因编码不同的蛋白质。
7. Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins. [O] . Schatz, P J, Pillus, L, Grisafi, P, 1986

机译：酵母酿酒酵母的两个功能性α-微管蛋白基因编码不同的蛋白质。
8. Cloning and Characterization of Genes Encoding Proteins Involved in the TerminalStage of Vesicular Traffic in the Yeast 'Saccharomyces cerevisiae' [R] . Aalto, M. 1995

机译：酵母'酿酒酵母'中水泡交通终末期蛋白质编码基因的克隆与鉴定

Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins.

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