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首页> 外文期刊>Molecular and Cellular Biology >Mitotic gene conversion lengths, coconversion patterns, and the incidence of reciprocal recombination in a Saccharomyces cerevisiae plasmid system.
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Mitotic gene conversion lengths, coconversion patterns, and the incidence of reciprocal recombination in a Saccharomyces cerevisiae plasmid system.

机译：酿酒酵母质粒系统中的有丝分裂基因转化长度，共转化模式和相互重组的发生率。

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Plasmids capable of undergoing genetic exchange in mitotically dividing Saccharomyces cerevisiae cells were used to measure the length of gene conversion events, to determine patterns of coconversion when multiple markers were present, and to correlate the incidence of reciprocal recombination with the length of conversion tracts. To construct such plasmids, restriction site linkers were inserted both within the HIS3 gene and in the flanking sequences, and two different his3- alleles were placed in a vector. Characterization of the genetic exchanges in these plasmids showed that most occur with the conversion of one his3- allele. Many of these events included coconversions in which more than one marker along the allelic sequence was replaced. The frequency of coconversion decreased with the distance between two markers such that markers further than 1 kilobase apart were infrequently coconverted. From these results the average length of conversion was determined to be approximately 0.5 kilobase. Examination of coconversions involving three or more markers revealed an almost obligatory, simultaneous coconversion pattern of all markers. Thus, when two markers which flank an intervening marker are converted, the intervening marker is 20 times more likely to be converted than to remain unchanged. The results of these studies also showed that the incidence of reciprocal recombination, which accompanies more than 20% of the conversion events, is more frequent when the conversion tract is longer than average.
机译：使用能够在有丝分裂的酿酒酵母细胞中进行遗传交换的质粒来测量基因转化事件的长度，确定存在多个标记物时的共转化模式，并使相互重组的发生率与转化道的长度相关。为了构建这样的质粒，将限制性位点接头插入HIS3基因内和侧翼序列中，并将两个不同的his3-等位基因置于载体中。这些质粒中遗传交换的表征表明，大多数发生在一个his3-等位基因转化的情况下。这些事件中有许多包括共转化，其中沿等位基因序列的一个以上标记已被替换。共转化的频率随着两个标记之间的距离而降低，因此相隔1公里以上的标记很少被共转化。根据这些结果，确定平均转化长度为约0.5kb。涉及三个或更多标记的共转化研究表明，所有标记几乎都是同时进行的共转化模式。因此，当转换位于中间标记两侧的两个标记时，该中间标记被转换的可能性比保持不变的可能性高20倍。这些研究的结果还表明，当转化率长于平均水平时，相互转化的发生率（超过20％的转化事件）更为频繁。

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