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首页> 外文期刊>Molecular and Cellular Biology >Multidrug resistance of DNA-mediated transformants is linked to transfer of the human mdr1 gene.
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Multidrug resistance of DNA-mediated transformants is linked to transfer of the human mdr1 gene.

机译：DNA介导的转化子的多药耐药性与人类mdr1基因的转移有关。

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摘要

Mouse NIH 3T3 cells were transformed to multidrug resistance with high-molecular-weight DNA from multidrug-resistant human KB carcinoma cells. The patterns of cross resistance to colchicine, vinblastine, and doxorubicin hydrochloride (Adriamycin; Adria Laboratories Inc.) of the human donor cell line and mouse recipients were similar. The multidrug-resistant human donor cell line contains amplified sequences of the mdr1 gene which are expressed at high levels. Both primary and secondary NIH 3T3 transformants contained and expressed these amplified human mdr1 sequences. Amplification and expression of the human mdr1 sequences and amplification of cotransferred human Alu sequences in the mouse cells correlated with the degree of multidrug resistance. These data suggest that the mdr1 gene is likely to be responsible for multidrug resistance in cultured cells.
机译：小鼠NIH 3T3细胞被来自具有多重耐药性的人KB癌细胞的高分子量DNA转化为具有多重耐药性。人类供体细胞系和小鼠受体对秋水仙碱，长春碱和盐酸阿霉素（阿德里亚霉素;阿德里亚实验室公司）的交叉抗性模式相似。耐多药的人类供体细胞系含有以高水平表达的mdr1基因的扩增序列。 NIH 3T3初级和次级转化子均包含并表达了这些扩增的人mdr1序列。小鼠细胞中人mdr1序列的扩增和表达以及共转移的人Alu序列的扩增与多药耐药程度相关。这些数据表明，mdr1基因可能是导致培养细胞多药耐药的原因。

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