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首页> 外文期刊>Molecular and Cellular Biology >Biosynthesis of the v-sis gene product: signal sequence cleavage, glycosylation, and proteolytic processing.
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Biosynthesis of the v-sis gene product: signal sequence cleavage, glycosylation, and proteolytic processing.

机译：v-sis基因产物的生物合成：信号序列切割，糖基化和蛋白水解过程。

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摘要

The v-sis oncogene and its cellular homolog c-sis encode chain B of platelet-derived growth factor. Cells transformed by v-sis produce a platelet-derived growth factor-related molecule which is able to stimulate the platelet-derived growth factor receptor in an autocrine fashion. Site-directed mutagenesis was used to construct several mutations which substitute charged residues for hydrophobic residues in the proposed signal sequence of the v-sis gene product. Two of these mutations resulted in the synthesis of altered v-sis gene products with an unexpected nuclear location and a loss of biological activity. We also report here the intracellular localization of the v-sis gene product to the endoplasmic reticulum-Golgi compartment, where signal sequence cleavage and N-linked glycosylation occur. The v-sis gene product contains no transmembrane regions, as it is completely protected within isolated microsomes from trypsin proteolysis. Site-directed mutagenesis was also used to alter a proposed proteolytic processing site in the v-sis gene product. This mutant v-sis gene, which encodes Asn-Ser in place of Lys-Arg at residues 110 to 111, was found to retain full biological activity.
机译：v-sis癌基因及其细胞同源c-sis编码血小板衍生生长因子的链B。通过v-sis转化的细胞产生了血小板衍生的生长因子相关分子，该分子能够以自分泌方式刺激血小板衍生的生长因子受体。使用定点诱变来构建多个突变，这些突变将v-sis基因产物的拟议信号序列中的带电荷残基替换为疏水残基。这些突变中的两个导致合成的v-sis基因产物发生了改变，具有意想不到的核位置和生物活性的丧失。我们在这里还报告了v-sis基因产物在细胞内定位到内质网-高尔基体的区域，在那里发生信号序列切割和N-联糖基化。 v-sis基因产物不包含跨膜区域，因为它在隔离的微粒体内完全受到胰蛋白酶水解的保护。定点诱变也用于改变v-sis基因产物中拟议的蛋白水解加工位点。发现该突变体v-sis基因在残基110至111处代替Lys-Arg编码Asn-Ser，其保留了完整的生物学活性。

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