Posttranscriptional control of human gamma interferon gene expression in transfected mouse fibroblasts.

H A Young; L Varesio; P Hwu

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Posttranscriptional control of human gamma interferon gene expression in transfected mouse fibroblasts.

机译：转录后控制人类γ干扰素基因在转染的小鼠成纤维细胞中的表达。

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Human gamma interferon genomic DNA was introduced into NIH 3T3 fibroblasts by calcium phosphate precipitation and was not expressed in these cells at the cytoplasmic mRNA or protein level. Treatment of the transfected cells with cycloheximide (1 microgram/ml) induced the accumulation of cytoplasmic gamma interferon mRNA and biologically active human gamma interferon. Analysis of the nuclear enriched RNA from untreated cells indicated that human gamma interferon mRNA was present, suggesting that cycloheximide may act by inhibiting a specific nuclease or may enhance the processing or transport of the RNA from the nucleus to the cytoplasm.

机译：人类γ干扰素基因组DNA通过磷酸钙沉淀被引入NIH 3T3成纤维细胞中，并且在细胞质mRNA或蛋白质水平上未在这些细胞中表达。用环己酰亚胺（1微克/毫升）处理转染的细胞诱导了细胞质γ干扰素mRNA和具有生物活性的人γ干扰素的积累。对来自未处理细胞的富核RNA的分析表明存在人γ干扰素mRNA，这表明环己酰亚胺可能通过抑制特定核酸酶而起作用，或者可以增强RNA从细胞核到细胞质的加工或转运。

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《Molecular and Cellular Biology》 |1986年第6期|共4页
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H A Young; L Varesio; P Hwu;
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中图分类细胞生物学;
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1. Regulation of Nuclear Gamma Interferon Gene Expression by Interleukin 12 (IL-12) and IL-2 Represents a Novel Form of Posttranscriptional Control [J] . Deborah L. Hodge, Alfredo Martinez, John G. Julias, Molecular and Cellular Biology . 2002,第6期

机译：白介素12（IL-12）和IL-2调节核γ干扰素基因表达代表转录后控制的一种新型形式。
2. Regulation of Nuclear Gamma Interferon Gene Expression by Interleukin 12 (IL-12) and IL-2 Represents a Novel Form of Posttranscriptional Control [J] . Deborah L. Hodge, Alfredo Martinez, John G. Julias, Molecular and Cellular Biology . 2002,第6期

机译：白介素12（IL-12）和IL-2调节核γ干扰素基因表达代表转录后控制的一种新型形式。
3. Interferon-gamma and interleukin 4 inhibit interleukin 1beta-induced delayed prostaglandin E(2)generation through suppression of cyclooxygenase-2 expression in human fibroblasts. [J] . Hayashi Y, Kobayashi M, Kuwata H, Cytokine . 2000,第6期

机译：干扰素-γ和白介素4抑制白介素1β诱导的延迟前列腺素E（2）生成通过抑制人类成纤维细胞中环氧合酶2表达。
4. Stably transfected human cell lines as fluorescent screening assay for Nuclear Factor κB activation dependent gene expression [C] . Christine E. Hellweg, Christa Baumstark-Khan, Gerda Horneck Conference on Genetically Engineered and Optical Probes for Biomedical Applications II; 20040124-20040127; San Jose,CA; US . 2004

机译：稳定转染的人类细胞系作为核因子κB激活依赖性基因表达的荧光筛选测定
5. Control of cytokine gene expression using antisense oligodeoxynucleotide: Blockade of interleukin-10 and interferon-gamma gene expression in pig cells. [D] . Sidahmed, Abubaker Mohamed Elhassan. 2003

机译：使用反义寡聚脱氧核苷酸控制细胞因子基因表达：猪细胞中白介素10和干扰素-γ基因表达的阻断。
6. Posttranscriptional control of human gamma interferon gene expression in transfected mouse fibroblasts. [O] . H A Young, L Varesio, P Hwu 1986

机译：转录后控制人类γ干扰素基因在转染的小鼠成纤维细胞中的表达。
7. Posttranscriptional control of human gamma interferon gene expression in transfected mouse fibroblasts. [O] . Young, H A, Varesio, L, Hwu, P 1986

机译：转录后控制人类γ干扰素基因在转染的小鼠成纤维细胞中的表达。
8. Gamma Interferon-Induced Expression of Class II MHC (Major Histocompability Complex) Antigens by Human Keratinocytes: Effects of Conditions of Culture [R] . Morhenn, V. B., Wood, G. S. 1987

机译：γ干扰素诱导人角质形成细胞表达II类mHC（主要组织相容性复合物）抗原：培养条件的影响

Posttranscriptional control of human gamma interferon gene expression in transfected mouse fibroblasts.

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