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首页> 外文期刊>Molecular and Cellular Biology >Antibodies to Xenopus egg S6 kinase II recognize S6 kinase from progesterone- and insulin-stimulated Xenopus oocytes and from proliferating chicken embryo fibroblasts.
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Antibodies to Xenopus egg S6 kinase II recognize S6 kinase from progesterone- and insulin-stimulated Xenopus oocytes and from proliferating chicken embryo fibroblasts.

机译：爪蟾卵S6激酶II的抗体从孕酮和胰岛素刺激的爪蟾卵母细胞和增殖的鸡胚成纤维细胞中识别S6激酶。

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摘要

Ribosomal protein S6 becomes highly phosphorylated during progesterone- or insulin-induced maturation of Xenopus laevis oocytes. We have previously purified an Mr 92,000 protein as one of the major S6 kinases from Xenopus unfertilized eggs. In this paper we confirm by renaturation of activity from a sodium dodecyl sulfate-polyacrylamide gel that this protein is an S6 kinase. This enzyme, termed S6 kinase II (S6 K II), was used for the preparation of polyclonal antiserum. Immunocomplexes formed with the antiserum and purified S6 K II were able to express kinase activity with the same substrate specificity as that of the purified enzyme, including autophosphorylation of S6 K II itself. The antiserum did not react with S6 kinase I, another major S6 kinase present in Xenopus eggs, which is chromatographically distinct from S6 K II. The administration of progesterone to oocytes resulted in a 20- to 25-fold increase in S6 kinase activity in extracts of these cells. Immunocomplex kinase assays done on extracts revealed that anti-S6 K II serum reacted with S6 kinase from progesterone-treated oocytes. This antiserum also reacted with the activated S6 kinase from insulin-stimulated oocytes. In addition, anti-S6 K II serum reacted with activated S6 kinase from chicken embryo fibroblasts stimulated with serum or transformed by Rous sarcoma virus. These results indicate that S6 K II or an antigenically related S6 kinase(s) is subject to regulation by mitogenic stimuli in various cell types.
机译：核糖体蛋白S6在黄体酮或胰岛素诱导的非洲爪蟾卵母细胞成熟过程中被高度磷酸化。我们以前从非洲爪蟾未受精卵中纯化了92,000先生蛋白，作为主要S6激酶之一。在本文中，我们通过对十二烷基硫酸钠-聚丙烯酰胺凝胶的活性进行复性来证实该蛋白是S6激酶。将该酶称为S6激酶II（S6 K II），用于制备多克隆抗血清。用抗血清和纯化的S6 K II形成的免疫复合物能够表达具有与纯化酶相同的底物特异性的激酶活性，包括S6 K II自身的自磷酸化。抗血清不与非洲爪蟾卵中存在的另一种主要的S6激酶S6激酶I反应，该色谱在色谱上不同于S6 K II。将黄体酮给予卵母细胞会使这些细胞提取物中的S6激酶活性提高20至25倍。对提取物进行的免疫复合物激酶测定表明，抗S6 K II血清与孕激素处理过的卵母细胞中的S6激酶反应。该抗血清也与来自胰岛素刺激的卵母细胞的活化的S6激酶反应。此外，抗-S6 K II血清与来自鸡胚成纤维细胞的活化S6激酶反应，所述鸡胚成纤维细胞被血清刺激或由劳斯肉瘤病毒转化。这些结果表明，在各种细胞类型中，S6KII或与抗原相关的S6激酶受到有丝分裂刺激的调节。

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