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首页> 外文期刊>Molecular and Cellular Biology >LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes.
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LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes.

机译：酿酒酵母的LEU3编码控制一组亮氨酸特异性基因RNA水平的因子。

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摘要

Although the majority of genes for amino acid biosynthesis which have been examined are under general amino acid control, LEU1 and LEU2 of Saccharomyces cerevisiae respond specifically to leucine. We report here an analysis of LEU3, a putative leucine-specific regulatory locus. We show that LEU3 is necessary for expression of wild-type levels of LEU1- and LEU2-specific RNAs and, further, that the levels of LEU4-specific transcripts are also affected by LEU3. We cloned LEU3 and showed by DNA sequence analysis that it contained an open reading frame of 886 amino acids. A striking feature of the predicted LEU3 protein was a cluster of acidic amino acids (19 of 20) located in the C-terminal half of the coding region. The protein also had a repeated cysteine motif which was conserved in a number of other yeast proteins implicated in gene regulation. We show that whole-cell extracts contained a LEU3-dependent DNA-binding activity that interacted with the 5' region of LEU2. Subdivision of the LEU2 5' region established that the LEU3-dependent DNA-binding activity interacted with the segment which had the previously reported homology with LEU1.
机译：尽管已检查的大多数氨基酸生物合成基因都在一般氨基酸控制下，但酿酒酵母的LEU1和LEU2对亮氨酸有特异性反应。我们在这里报告了LEU3（一种推测的亮氨酸特异性调控位点）的分析。我们表明，LEU3是表达野生型水平的LEU1和LEU2特异性RNA所必需的，此外，LEU4特异性转录物的水平也受到LEU3的影响。我们克隆了LEU3，并通过DNA序列分析显示它含有886个氨基酸的开放阅读框。预测的LEU3蛋白的显着特征是位于编码区C端一半的酸性氨基酸簇（20个中的19个）。该蛋白质还具有重复的半胱氨酸基序，在基因调控中涉及的许多其他酵母蛋白质中均是保守的。我们显示全细胞提取物包含与LEU2的5'区域相互作用的LEU3依赖性DNA结合活性。对LEU2 5'区的细分表明，依赖LEU3的DNA结合活性与先前报道与LEU1具有同源性的区段相互作用。

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