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首页> 外文期刊>Molecular and Cellular Biology >The stability of mRNA for eucaryotic elongation factor Tu in Friend erythroleukemia cells varies with growth rate.
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The stability of mRNA for eucaryotic elongation factor Tu in Friend erythroleukemia cells varies with growth rate.

机译：Friend红白血病细胞中真核细胞延伸因子Tu的mRNA稳定性随生长速率而变化。

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The decay rates of eucaryotic elongation factor Tu (eEF-Tu) mRNA and eucaryotic initiation factor 4A (eIF-4A) mRNA in Friend erythroleukemia (FEL) cells were determined under several different growth conditions. In FEL cells which were no longer actively dividing (stationary phase), eEF-Tu mRNA was found to be rather stable, with a t1/2 of about 24 h. In rapidly growing FEL cells eEF-Tu mRNA was considerably less stable, with a t1/2 of about 9 h. In both cases a single rate of mRNA decay was observed. However, when stationary-phase cells resumed growth after treatment with fresh medium, we observed that eEF-Tu mRNA decay followed a biphasic process. The faster of the two decay rates involved approximately 50% of the eEF-Tu mRNA and had a t1/2 of about 1 h. The decay rates for eIF-4A (t1/2 = 2 h) and total poly(A)+ RNA (t1/2 = 3 h) were unaffected by changes in growth conditions. The t1/2 for polysomal eEF-Tu mRNA was found to be about 8 h when stationary FEL cells were treated with fresh medium. Previous work in this laboratory has shown (T. R. Rao and L. I. Slobin, Mol. Cell. Biol. 7:687-697, 1987) that when FEL cells are allowed to grow to stationary phase, approximately 60% of the mRNA for eEF-Tu is found in a nontranslating postpolysomal messenger ribonucleoprotein (mRNP) particle. eEF-Tu mRNP was rapidly cleared from stationary cells after treatment with fresh medium. The data presented in this report indicate that the stability of eEF-Tu mRNP is rapidly altered and the particle is targeted for degradation when stationary FEL cells resume growth.
机译：在几种不同的生长条件下，确定了Friend erythreuemiaemia（FEL）细胞中的真核延伸因子Tu（eEF-Tu）mRNA和真核起始因子4A（eIF-4A）mRNA的衰减率。在不再活跃分裂（静止期）的FEL细胞中，发现eEF-Tu mRNA相当稳定，t1 / 2约为24小时。在快速生长的FEL细胞中，eEF-Tu mRNA的稳定性大大降低，t1 / 2约为9小时。在这两种情况下，均观察到mRNA衰减的单一速率。但是，当固定相细胞在用新鲜培养基处理后恢复生长时，我们观察到eEF-Tu mRNA的衰变遵循双相过程。两种衰变速率中较快的约占eEF-Tu mRNA的50％，t1 / 2约为1小时。 eIF-4A（t1 / 2 = 2 h）和总poly（A）+ RNA（t1 / 2 = 3 h）的衰减率不受生长条件变化的影响。当用新鲜培养基处理固定的FEL细胞时，发现多体eEF-Tu mRNA的t1 / 2约为8小时。该实验室先前的工作表明（TR Rao和LI Slobin，分子细胞生物学，7：687-697，1987）表明，当允许FEL细胞生长至固定相时，eEF-Tu的mRNA约占60％在非翻译后的多体信使核糖核酸蛋白（mRNP）颗粒中发现了这种蛋白。用新鲜培养基处理后，eEF-Tu mRNP迅速从静止细胞中清除。该报告中提供的数据表明，当固定的FEL细胞恢复生长时，eEF-Tu mRNP的稳定性迅速改变，并且该颗粒被靶向降解。

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