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首页> 外文期刊>Molecular and Cellular Biology >Translational control mediated by eucaryotic initiation factor-2 is restricted to specific mRNAs in transfected cells.
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Translational control mediated by eucaryotic initiation factor-2 is restricted to specific mRNAs in transfected cells.

机译：由氨基酸酯引发因子-2介导的翻译控制仅限于转染细胞中的特异性mRNA。

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摘要

The translational efficiency of mRNA molecules transcribed from plasmid DNA transfected into COS-1 monkey cells can be increased 10- to 20-fold by the coexpression of the adenovirus virus-associated RNAs I and II. Experiments described here demonstrate a similar increase in translational efficiency by the addition of 2-aminopurine, an inhibitor of double-stranded RNA-activated protein kinase, to the culture medium. Both virus-associated RNA and 2-aminopurine presumably exert their effect by alteration of the functional level of eucaryotic initiation factor-2. The translational stimulation mediated by both means is shown to be restricted to the plasmid-derived mRNAs because there is no qualitative or quantitative alteration in host protein synthesis. The results are consistent with models invoking a localized activation of double-stranded RNA-activated kinase leading to a translational block.
机译：通过转染到COS-1猴细胞转染的质粒DNA转录的mRNA分子的平移效率可以通过腺病毒病毒相关的RNA I和II的共表达增加10-20倍。这里描述的实验证明了通过加入2-氨基嘌呤，将双链RNA活化蛋白激酶的抑制剂，对培养基相似的平移效率的增加。病毒相关的RNA和2-氨基嘌呤均可能通过改变核酸氨基因引发因子-2的功能水平来施加它们的效果。两种方法介导的平移刺激被证明限于质粒衍生的MRNA，因为在宿主蛋白质合成中没有没有定性或定量的改变。结果与调用局部激活的双链RNA活化激酶导致平移块的模型一致。

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