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首页> 外文期刊>Molecular and Cellular Biology >Mating-type control in Saccharomyces cerevisiae: isolation and characterization of mutants defective in repression by a1-alpha 2.
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Mating-type control in Saccharomyces cerevisiae: isolation and characterization of mutants defective in repression by a1-alpha 2.

机译：酿酒酵母中的交配型控制：a1-alpha 2阻遏缺陷的突变体的分离和表征。

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The alpha 2 protein, the product of the MAT alpha 2 cistron, represses various genes specific to the a mating type (alpha 2 repression), and when combined with the MATa1 gene product, it represses MAT alpha 1 and various haploid-specific genes (a1-alpha 2 repression). One target of a1-alpha 2 repression is RME1, which is a negative regulator of a/alpha-specific genes. We have isolated 13 recessive mutants whose a1-alpha 2 repression is defective but which retain alpha 2 repression in a genetic background of ho MATa HML alpha HMRa sir3 or ho MAT alpha HMRa HMRa sir3. These mutations can be divided into three different classes. One class contains a missense mutation, designated hml alpha 2-102, in the alpha 2 cistron of HML, and another class contains two mat alpha 2-202, in the MAT alpha locus. These three mutants each have an amino acid substitution of tyrosine or acid substitution of tyrosine or phenylalanine for cysteine at the 33rd codon from the translation initiation codon in the alpha 2 cistron of HML alpha or MAT alpha. The remaining 10 mutants make up the third class and form a single complementation group, having mutations designated aar1 (a1-alpha 2 repression), at a gene other than MAT, HML, HMR, RME1, or the four SIR genes. Although a diploid cell homozygous for the aarl and sir3 mutations and for the MATa, HML alpha, and HMRa alleles showed alpha mating type, it could sporulate and gave rise to asci containing four alpha mating-type spores. These facts indicate that the domain for alpha2 repression is separable from that for a1-alpha2 protein interaction or complex formation in the alpha2 protein and that an additional regulation gene, AAR1, is associated with the a1-alpha2 repression of the alpha1 cistron and haploid-specific genes.
机译：MAT alpha 2顺反子的产物alpha 2蛋白可抑制多种特定于交配类型的基因（alpha 2抑制），当与MATa1基因产物结合后，它将抑制MAT alpha 1和多种单倍体特异性基因（ a1-alpha 2抑制）。 RME1是α1-α2抑制的一个目标，它是α/α特异性基因的负调控因子。我们已经分离出13个隐性突变体，它们的a1-alpha 2抑制是有缺陷的，但是在ho MATa HML alpha HMRa sir3或ho MAT alpha HMRa HMRa sir3的遗传背景中保留了alpha 2抑制。这些突变可分为三个不同的类别。一类在HML的alpha 2顺反子中包含一个错义突变，称为hml alpha 2-102，另一类在MAT alpha座位中包含两个matα2-202。这三个突变体各自在HML alpha或MAT alpha alpha 2顺反子的翻译起始密码子的第33个密码子处具有酪氨酸的氨基酸取代或酪氨酸或苯丙氨酸的酸取代半胱氨酸。其余的10个突变体构成第三类，并形成一个互补组，在MAT，HML，HMR，RME1或4个SIR基因以外的其他基因上具有被命名为aar1（a1-alpha 2抑制）的突变。尽管对于aarl和sir3突变以及MATa，HMLα和HMRa等位基因的二倍体细胞是纯合的，但它们可以形成孢子并产生包含四个alpha交配型孢子的asci。这些事实表明，alpha2阻遏域与a1-alpha2蛋白相互作用或在alpha2蛋白中形成复合物的域是可分离的，另外一个调控基因AAR1与alpha1顺反子和单倍体的a1-alpha2阻遏有关。特定基因。

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