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首页> 外文期刊>Molecular and Cellular Biology >KEX2 mutations suppress RNA polymerase II mutants and alter the temperature range of yeast cell growth.
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KEX2 mutations suppress RNA polymerase II mutants and alter the temperature range of yeast cell growth.

机译：KEX2突变抑制RNA聚合酶II突变，并改变酵母细胞生长的温度范围。

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摘要

Suppressors of a temperature-sensitive RNA polymerase II mutation were isolated to identify proteins that interact with RNA polymerase II in yeast cells. Ten independently isolated extragenic mutations that suppressed the temperature-sensitive mutation rpb1-1 and produced a cold-sensitive phenotype were all found to be alleles of a single gene, SRB1. An SRB1 partial deletion mutant was further investigated and found to exhibit several pleiotropic phenotypes. These included suppression of numerous temperature-sensitive RNA polymerase II mutations, alteration of the temperature growth range of cells containing wild-type RNA polymerase, and sterility of cells of alpha mating type. The ability of SRB1 mutations to suppress the temperature-sensitive phenotype of RNA polymerase II mutants did not extend to other temperature-sensitive mutants investigated. Isolation of the SRB1 gene revealed that SRB1 is KEX2. These results indicate that the KEX2 protease, whose only known substrates are hormone precursors, can have an important influence on RNA polymerase II and the temperature-dependent growth properties of yeast cells.
机译：分离出对温度敏感的RNA聚合酶II突变的抑制子，以鉴定与酵母细胞中的RNA聚合酶II相互作用的蛋白质。发现十个独立分离的外源突变，它们抑制了对温度敏感的突变rpb1-1并产生了冷敏感的表型，都是单基因SRB1的等位基因。 SRB1部分删除突变体进行了进一步研究，发现表现出几种多效性的表型。这些措施包括抑制许多对温度敏感的RNA聚合酶II突变，改变含有野生型RNA聚合酶的细胞的温度生长范围，以及阿尔法交配型细胞的无菌性。 SRB1突变抑制RNA聚合酶II突变体的温度敏感表型的能力没有扩展到其他调查的温度敏感突变体。 SRB1基因的分离显示SRB1是KEX2。这些结果表明，仅已知底物为激素前体的KEX2蛋白酶可对RNA聚合酶II和酵母细胞的温度依赖性生长特性产生重要影响。

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