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首页> 外文期刊>Molecular and Cellular Biology >Role of phosphatidylinositide metabolism in ras-induced Xenopus oocyte maturation.
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Role of phosphatidylinositide metabolism in ras-induced Xenopus oocyte maturation.

机译：磷脂酰肌醇代谢在ras诱导的非洲爪蟾卵母细胞成熟中的作用。

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摘要

Microinjection of Xenopus oocytes with ras protein (p21) was used to investigate the role of phospholipid metabolism in ras-induced meiotic maturation. Induction of meiosis by ras was compared with induction by progesterone, insulin, and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Neomycin, which specifically binds to phosphatidylinositides and inhibits their metabolism, blocked meiotic maturation induced by ras or insulin but not by progesterone or TPA. In addition, p21 and TPA, but not insulin or progesterone, stimulated the incorporation of 32Pi into oocyte lipids. ras protein specifically stimulated 32P incorporation into phosphatidylinositides, whereas both ras and TPA stimulated 32P incorporation into phosphatidylcholine and phosphatidylethanolamine. The stimulatory effect of p21 on phosphatidylinositide metabolism correlated with the dose response and kinetics of ras-induced meiotic maturation. In addition, the ras oncogene protein was more potent than the proto-oncogene protein both in inducing meiotic maturation and in stimulating phosphatidylinositide metabolism. These results indicate that phosphatidylinositide turnover is required for ras-induced meiosis and suggest that phosphatidylinositide-derived second messengers mediate the biological activity of ras in Xenopus oocytes.
机译：用ras蛋白（p21）显微注射非洲爪蟾卵母细胞来研究磷脂代谢在ras诱导的减数分裂成熟中的作用。将ras诱导的减数分裂与孕酮，胰岛素和佛波酯12-O-十四烷酰佛波13-乙酸酯（TPA）的诱导进行了比较。新霉素可特异性结合磷脂酰肌醇并抑制其代谢，可阻断ras或胰岛素诱导的减数分裂成熟，但不会抑制孕激素或TPA诱导的减数分裂成熟。另外，p21和TPA而不是胰岛素或孕酮刺激了32Pi掺入卵母细胞脂质中。 ras蛋白可特异性刺激32P掺入磷脂酰肌醇，而ras和TPA均可刺激32P掺入磷脂酰胆碱和磷脂酰乙醇胺。 p21对磷脂酰肌醇代谢的刺激作用与ras诱导的减数分裂成熟的剂量反应和动力学有关。此外，ras癌基因蛋白在诱导减数分裂成熟和刺激磷脂酰肌醇代谢方面均比原癌基因蛋白更有效。这些结果表明磷脂酰肌醇肽转换是ras诱导的减数分裂所必需的，并且表明磷脂酰肌醇肽衍生的第二信使介导了非洲爪蟾卵母细胞中ras的生物学活性。

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