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首页> 外文期刊>Molecular and Cellular Biology >Translational control of discoidin lectin expression in drsA suppressor mutants of Dictyostelium discoideum.
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Translational control of discoidin lectin expression in drsA suppressor mutants of Dictyostelium discoideum.

机译：盘基网柄菌的drsA抑制突变体中盘基凝集素表达的翻译控制。

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Genetic analysis in Dictyostelium discoideum has identified regulatory genes which control the developmental expression of the discoidin lectin multigene family. Among these, the drsA mutation is a dominant second-site suppressor of another mutation, disB, which has the discoidinless phenotype. We now demonstrate a novel mechanism by which the drsA allele exerts its suppressive effect on the disB mutation. Interestingly, drsA does not merely bypass the disB mutation and restore the wild-type pattern of lectin expression. Rather, drsA mutant cells have high levels of discoidin lectin synthesis during growth but do not express lectins during aggregation. In contrast, wild-type cells only express lectin protein during the aggregation period of development. Phenocopies of the drsA mutation show a pattern of discoidin expression similar to that seen in the bona fide mutant. These data suggest that there may be a mechanism of negative feedback, resulting from the high levels of discoidin lectin made during growth, which inhibits further discoidin lectin expression during development. Northern (RNA) analysis of developing drsA mutant cells shows that these cells contain high levels of discoidin mRNA, although no discoidin lectin protein is being translated from these messages. Therefore, expression of the discoidin gene family can be controlled at the level of translation as well as transcription.
机译：盘基网柄菌的遗传分析已鉴定出调节盘状凝集素凝集素多基因家族的发育表达的调控基因。其中，drsA突变是另一突变disB的主要第二位抑制剂，disB具有无盘状表型。现在，我们证明了一种新颖的机制，通过该机制，drsA等位基因可对disB突变发挥抑制作用。有趣的是，drsA不仅绕过了disB突变并恢复了凝集素表达的野生型模式。而是，drsA突变细胞在生长过程中具有高水平的盘状凝集素凝集素合成，但在聚集过程中不表达凝集素。相反，野生型细胞仅在发育的聚集期表达凝集素蛋白。 drsA突变的表型显示盘状蛋白表达的模式类似于在真正的突变体中看到的。这些数据表明，可能由于生长过程中产生的盘状凝集素凝集素水平高而导致负反馈的机制，这会抑制发育过程中盘状凝集素凝集素的进一步表达。对发育中的drsA突变细胞的Northern（RNA）分析表明，尽管这些信息中没有任何discodisin凝集素蛋白被翻译出来，但这些细胞中含有高水平的discodisin mRNA。因此，盘状蛋白基因家族的表达可以在翻译和转录水平上得到控制。

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