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首页> 外文期刊>Molecular and Cellular Biology >A suppressor of temperature-sensitive rna mutations that affect mRNA metabolism in Saccharomyces cerevisiae.
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A suppressor of temperature-sensitive rna mutations that affect mRNA metabolism in Saccharomyces cerevisiae.

机译：温度敏感性rna突变的抑制剂，可影响酿酒酵母中的mRNA代谢。

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摘要

We have isolated a dominant suppressor of rna mutation (SRN1) that relieves the temperature-sensitive inhibition of mRNA synthesis of ribosomal protein genes in the yeast Saccharomyces cerevisiae. The suppressor was selected for its ability to alleviate simultaneously the temperature-sensitive growth phenotypes of rna2 and rna6. Several independently isolated suppressors appeared to be recessive lethal mutations. One suppressor, SRN1, was recovered as viable in haploid strains. SRN1 can suppress rna2, rna3, rna4, rna5, rna6, and rna8 singly or in pairs, although some combinations of rna mutations are less well suppressed than others. The suppressor allows strains with rna mutations to grow at 34 degrees C but is unable to suppress at 37 degrees C; however, SRN1 does not, by itself, prevent growth at 37 degrees C. In addition, SRN1 suppresses the rna1 mutation which affects general mRNA levels and also leads to the accumulation of precursor tRNA for those tRNAs that have intervening sequences. SRN1 can suppress the rna1 mutation as well as the rna1 rna2 double mutation at 34 degrees C. The suppressor does not affect the temperature-sensitive growth of two unrelated temperature-sensitive mutations, cdc4 and cdc7.
机译：我们已经分离出一个主要的rna突变抑制因子（SRN1），该抑制剂可缓解酿酒酵母中核糖体蛋白基因的mRNA合成的温度敏感性抑制。选择抑制因子是因为其能够同时缓解rna2和rna6对温度敏感的生长表型。几种独立分离的抑制子似乎是隐性致死突变。一种抑制物SRN1在单倍体菌株中被回收。 SRN1可以单独或成对抑制rna2，rna3，rna4，rna5，rna6和rna8，尽管rna突变的某些组合受抑制程度较其他组合低。该抑制子使具有rna突变的菌株能够在34摄氏度下生长，但无法在37摄氏度下进行抑制。但是，SRN1本身并不能阻止其在37摄氏度下生长。此外，SRN1抑制了rna1突变，该突变影响总体mRNA水平，并导致具有中间序列的tRNA积累了前体tRNA。 SRN1可以在34摄氏度下抑制rna1突变以及rna1 rna2双重突变。该抑制剂不会影响两个不相关的温度敏感突变cdc4和cdc7的温度敏感生长。

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