Production of human c-myc protein in insect cells infected with a baculovirus expression vector.

C Miyamoto; G E Smith; J Farrell-Towt; R Chizzonite; M D Summers; G Ju

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Production of human c-myc protein in insect cells infected with a baculovirus expression vector.

机译：在杆状病毒表达载体感染的昆虫细胞中产生人c-myc蛋白。

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A cDNA fragment coding for human c-myc was inserted into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus adjacent to the strong polyhedrin promoter. Insect cells infected with the recombinant virus produced significant amounts of c-myc protein, which constituted the major phosphoprotein component in these cells. By immunoprecipitation and immunoblot analysis, two proteins of 61 and 64 kilodaltons were detected with c-myc-specific antisera. The insect-derived proteins were compared with recombinant human c-myc-encoded proteins synthesized in Escherichia coli and Saccharomyces cerevisiae cells. The c-myc gene product was found predominantly in the nucleus by subcellular fractionation of infected insect cells.

机译：编码人c-myc的cDNA片段被插入到杆状病毒Autographa californica核多角体病毒的基因组中，与强多角体蛋白启动子相邻。感染重组病毒的昆虫细胞产生大量c-myc蛋白，其构成了这些细胞中的主要磷蛋白成分。通过免疫沉淀和免疫印迹分析，使用c-myc特异性抗血清检测到61和64道尔顿的两种蛋白质。将昆虫来源的蛋白质与在大肠杆菌和酿酒酵母细胞中合成的重组人c-myc编码蛋白质进行比较。 c-myc基因产物主要是通过感染昆虫细胞的亚细胞分级分离而在细胞核中发现的。

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《Molecular and Cellular Biology》 |1985年第10期|共6页
作者
C Miyamoto; G E Smith; J Farrell-Towt; R Chizzonite; M D Summers; G Ju;
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中图分类细胞生物学;
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专利

1. Production of human beta interferon in insect cells infected with a baculovirus expression vector. [J] . G E Smith, M D Summers, M J Fraser Molecular and Cellular Biology . 1983,第12期

机译：在感染杆状病毒表达载体的昆虫细胞中产生人β干扰素。
2. Production of human beta interferon in insect cells infected with a baculovirus expression vector. [J] . G E Smith, M D Summers, M J Fraser Molecular and Cellular Biology . 1983,第12期

机译：在感染杆状病毒表达载体的昆虫细胞中产生人β干扰素。
3. Expression of a membrane-bound form of Trypanosoma cruzi trans-sialidase in baculovirus-infected insect cells: a potential tool for sialylation of glycoproteins produced in the baculovirus-insect cells system. [J] . Marchal I, Cerutti M, Mir AM, Glycobiology. . 2001,第7期

机译：杆状病毒感染的昆虫细胞中膜结合形式的克氏锥虫转唾液酸酶的表达：杆状病毒-昆虫细胞系统中产生的糖蛋白唾液酸化的潜在工具。
4. Expression and characterization of mouse epidermal transglutaminase in baculovirus-infected insect cells [C] . Kiyotaka Hitomi, Koji Ikura, Masatoshi Maki Joint International Meeting of the Japanese Association for Animal Cell Technolog and and European Society for Animal Cell Technology . 1999

机译：小鼠表皮转谷氨酰胺酶在杆状病毒感染昆虫细胞中的表达及表征
5. Effect of simulated microgravity conditions on BTI-Tn5B1-4 insect cell metabolism, virus infection, and protein production in the baculovirus expression vector system. [D] . Hollar, Kathryn Ann. 2001

机译：模拟微重力条件对杆状病毒表达载体系统中BTI-Tn5B1-4昆虫细胞代谢，病毒感染和蛋白质产生的影响。
6. Production of human c-myc protein in insect cells infected with a baculovirus expression vector. [O] . C Miyamoto, G E Smith, J Farrell-Towt, 1985

机译：在感染杆状病毒表达载体的昆虫细胞中产生人c-myc蛋白。
7. Production of human c-myc protein in insect cells infected with a baculovirus expression vector. [O] . Miyamoto, C, Smith, G E, Farrell-Towt, J, 1985

机译：在感染杆状病毒表达载体的昆虫细胞中产生人c-myc蛋白。

Production of human c-myc protein in insect cells infected with a baculovirus expression vector.

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