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首页> 外文期刊>Molecular and Cellular Biology >Production of human beta interferon in insect cells infected with a baculovirus expression vector.
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Production of human beta interferon in insect cells infected with a baculovirus expression vector.

机译：在感染杆状病毒表达载体的昆虫细胞中产生人β干扰素。

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Autographa californica nuclear polyhedrosis virus (AcNPV) was used as an expression vector for human beta interferon. By using specially constructed plasmids, the protein-coding sequences for interferon were linked to the AcNPV promoter for the gene encoding for polyhedrin, the major occlusion protein. The interferon gene was inserted at various locations relative to the AcNPV polyhedrin transcriptional and translational signals, and the interferon-polyhedrin hybrid genes were transferred to infectious AcNPV expression vectors. Biologically active interferon was produced, and greater than 95% was secreted from infected insect cells. A maximum of ca. 5 X 10(6) U of interferon activity was produced by 10(6) infected cells. These results demonstrate that AcNPV should be suitable for use as a eucaryotic expression vector for the production of products from cloned genes.
机译：加利福尼亚州卷柏核多角体病毒（AcNPV）被用作人类β干扰素的表达载体。通过使用特殊构建的质粒，干扰素的蛋白质编码序列与AcNPV启动子相连，该启动子编码主要的结合蛋白多角体蛋白。将干扰素基因插入到相对于AcNPV多角体蛋白转录和翻译信号的各个位置，然后将干扰素-多角体蛋白杂种基因转移到传染性AcNPV表达载体上。产生了具有生物活性的干扰素，感染的昆虫细胞分泌了95％以上的干扰素。最多约。 5 X 10（6）U的干扰素活性是由10（6）感染的细胞产生的。这些结果表明，AcNPV应适合用作真核表达载体，用于从克隆的基因生产产物。

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