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首页> 外文期刊>Molecular and Cellular Biology >Circular and linear simian virus 40 DNAs differ in recombination.
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Circular and linear simian virus 40 DNAs differ in recombination.

机译：环状和线性猿猴病毒40个DNA的重组不同。

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摘要

Linear forms of simian virus 40 (SV40) DNA, when added to transfection mixtures containing circular SV40 and phi X174 RFI DNAs, enhanced the frequency of SV40/phi X174 recombination, as measured by infectious center in situ plaque hybridization in monkey BSC-1 cells. The sequences required for the enhancement of recombination by linear DNA reside within the SV40 replication origin/regulatory region (nucleotides 5,171 to 5,243/0 to 128). Linearization of phi X174 RFI DNA did not increase the recombination frequency. The SV40/phi X174 recombinant structures arising from transfections supplemented with linear forms of origin-containing SV40 DNA contained phi X174 DNA sequences interspersed within tandem head-to-tail repeats derived from the recombination-enhancing linear DNA. Evidence is presented that the tandem repeats are not formed by homologous recombination and that linear forms of SV40 DNA must compete with circular SV40 DNA for the available T antigen to enhance recombination. We propose that the enhancement of recombination by linear SV40 DNA results from the entry of that DNA into a rolling circle type of replication pathway which generates highly recombinogenic intermediates.
机译：通过将线性形式的猿猴病毒40（SV40）DNA添加到包含环状SV40和phi X174 RFI DNA的转染混合物中，可以提高SV40 / phi X174重组的频率，这是通过猴BSC-1细胞中的感染性原位斑块杂交测定的。线性DNA增强重组所需的序列位于SV40复制起点/调控区（核苷酸5,171至5,243 / 0至128）内。 phi X174 RFI DNA的线性化未增加重组频率。由转染产生的SV40 / phi X174重组结构补充了线性形式的含起点的SV40 DNA，其中包含phi X174 DNA序列，这些序列散布在源自重组增强线性DNA的串联头对尾重复序列中。证据表明串联重复序列不是通过同源重组形成的，线性形式的SV40 DNA必须与环状SV40 DNA竞争可用的T抗原以增强重组。我们建议线性SV40 DNA增强重组是由于该DNA进入滚环型复制途径而产生的，该途径产生高度重组的中间体。

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