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首页> 外文期刊>Molecular and Cellular Biology >Identification of a sequence element on the 3' side of AAUAAA which is necessary for simian virus 40 late mRNA 3'-end processing.
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Identification of a sequence element on the 3' side of AAUAAA which is necessary for simian virus 40 late mRNA 3'-end processing.

机译：鉴定AAUAAA 3'侧的序列元件，该序列元件是猿猴病毒40晚期mRNA 3'末端加工所必需的。

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Our previous studies of the 3'-end processing of simian virus 40 late mRNAs indicated the existence of an essential element (or elements) downstream of the AAUAAA signal. We report here the use of transient expression analysis to study a functional element which we located within the sequence AGGUUUUUU, beginning 59 nucleotides downstream of the recognized signal AAUAAA. Deletion of this element resulted in (i) at least a 75% drop in 3'-end processing at the normal site and (ii) appearance of readthrough transcripts with alternate 3' ends. Some flexibility in the downstream position of this element relative to the AAUAAA was noted by deletion analysis. Using computer sequence comparison, we located homologous regions within downstream sequences of other genes, suggesting a generalized sequence element. In addition, specific complementarity is noted between the downstream element and U4 RNA. The possibility that this complementarity could participate in 3'-end site selection is discussed.
机译：我们先前对猿猴病毒40个晚期mRNA的3'末端加工的研究表明，AAUAAA信号下游存在一种或多种必需元素。我们在这里报告使用瞬时表达分析来研究一个功能元件，该功能元件位于序列AGGUUUUUU中，起始于识别信号AAUAAA下游59个核苷酸。删除该元素导致（i）在正常位点的3'末端加工过程至少下降了75％，并且（ii）出现了具有交替3'末端的通读转录本。通过缺失分析指出了该元件相对于AAUAAA的下游位置的一些灵活性。使用计算机序列比较，我们在其他基因的下游序列中定位了同源区域，表明了一个通用的序列元件。另外，在下游元件和U4 RNA之间发现了特定的互补性。讨论了这种互补性可能参与3'末端位点选择的可能性。

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