...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Estrogen regulation of the avian transferrin gene in transgenic mice.
【24h】

Estrogen regulation of the avian transferrin gene in transgenic mice.

机译：转基因小鼠中禽转铁蛋白基因的雌激素调节。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The intact chicken transferrin gene was microinjected into fertilized mouse eggs, and the resulting transgenic animals were used to produce lines of mice containing integrated copies of the chicken gene. The levels of expression of the chicken gene were quantitated in various tissues, and the response of the gene to estrogen stimulation was measured after chronic or acute estrogen exposure. Two of the three mouse lines studied maintained stable levels of expression in successive generations of offspring, and the third line had two- to threefold-higher levels in offspring than in the original parent. In the third line, the original transgenic parent was found to be a mosaic. The chicken transferrin gene was expressed at 10- to 20-fold-higher levels in liver than in any other tissue; however, the levels of chicken transferrin mRNA in kidney were higher than expected, indicating that the tissue specificity was only partial. In all three lines, the foreign gene was induced by estrogen administration. After 10 days of estrogen administration, there was a twofold increase in both transferrin mRNA and transcription of the chicken transferrin gene. A single injection of estradiol led to a fourfold increase in transferrin mRNA synthesis at 4h. As a control the levels of mouse albumin were measured, and both the level of albumin mRNA and its rate of transcription declined about twofold after estrogen administration. Our results indicate that the intact chicken gene with 2.2 kilobases of 5' flanking sequence contains signals for both tissue specificity and steroid regulation that can be recognized in mice.
机译：将完整的鸡转铁蛋白基因显微注射到受精的小鼠卵中，并将所得的转基因动物用于产生含有鸡基因整合拷贝的小鼠品系。在各种组织中定量鸡基因的表达水平，并在长期或急性暴露于雌激素后测量该基因对雌激素刺激的反应。研究的三个小鼠品系中的两个在后代的后代中保持稳定的表达水平，而第三个品系在后代中的表达水平比原始亲本高两到三倍。在第三行中，发现原始的转基因亲本是镶嵌体。鸡的转铁蛋白基因在肝脏中的表达水平比其他任何组织高10至20倍。然而，肾脏中鸡运铁蛋白的mRNA水平高于预期，表明组织特异性仅是部分的。在所有三个系中，外源基因是通过雌激素给药诱导的。服用雌激素10天后，运铁蛋白mRNA和鸡运铁蛋白基因的转录均增加了两倍。一次注射雌二醇在4h时导致转铁蛋白mRNA合成增加了四倍。作为对照，测量了小鼠白蛋白的水平，并且在施用雌激素后，白蛋白mRNA的水平及其转录速率均下降了约两倍。我们的结果表明，完整的鸡基因具有2.2千个碱基的5'侧翼序列，包含可在小鼠中识别的组织特异性和类固醇调节信号。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号