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首页> 外文期刊>Molecular and Cellular Biology >Two developmental stages of Neurospora crassa utilize similar mechanisms for responding to heat shock but contrasting mechanisms for recovery.
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Two developmental stages of Neurospora crassa utilize similar mechanisms for responding to heat shock but contrasting mechanisms for recovery.

机译：景天孢的两个发育阶段利用相似的机制来应对热激，但是利用相反的机制来恢复。

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At the heat shock temperature of 45 degrees C, there is a transient induction of the synthesis of heat shock proteins and repression of normal protein synthesis in cells of Neurospora crassa. Both conidiospores and mycelial cells resume normal protein synthesis after 60 min at high temperature. At the RNA level, however, these two developmental stages responded with different kinetics to elevated temperature. Heat shock RNAs (for hsp30 and hsp83) accumulated and declined more rapidly in spores than in mycelia, and during recovery spores accumulated mRNA that encoded a normal protein (the proteolipid subunit of the mitochondrial ATPase), whereas mycelia showed no increase in this normal RNA (for at least 120 min). Therefore, the resumption of normal protein synthesis in spores may depend upon accumulation of new mRNAs. In contrast, mycelial cells appeared to change their translational preference during continued incubation at elevated temperature, from a discrimination against normal mRNAs to a resumption of their translation into normal cellular proteins, exemplified by the ATPase proteolipid subunit whose synthesis was measured in the heat-shocked cells.
机译：在45度的热激温度下，神经孢菌的细胞中会短暂诱导热激蛋白的合成并抑制正常蛋白的合成。高温下60分钟后，分生孢子和菌丝体细胞都恢复了正常的蛋白质合成。然而，在RNA水平上，这两个发育阶段以不同的动力学响应于高温。热休克RNA（针对hsp30和hsp83）在孢子中的积累和下降比菌丝体更快，在恢复过程中，孢子积累的mRNA编码正常蛋白（线粒体ATPase的蛋白脂亚基），而菌丝体在正常RNA中没有增加（至少120分钟）。因此，孢子中正常蛋白质合成的恢复可能取决于新mRNA的积累。相比之下，菌丝细胞似乎在高温持续孵育过程中改变了翻译偏好，从对正常mRNA的识别到恢复为正常细胞蛋白的翻译，以ATPase蛋白脂亚基为例，其合成是在热激中测定的细胞。

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