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首页> 外文期刊>Molecular and Cellular Biology >Signal-dependent translocation of simian virus 40 large-T antigen into rat liver nuclei in a cell-free system.
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Signal-dependent translocation of simian virus 40 large-T antigen into rat liver nuclei in a cell-free system.

机译：猿猴病毒40大T抗原在无细胞系统中的信号依赖性转位进入大鼠肝核。

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摘要

An in vitro nuclear translocation system is described in which isolated rat liver nuclei were incubated in a defined buffered medium containing radiolabeled or fluorescently labeled exogenous proteins. The nuclei were rapidly recovered, extracted, and analyzed for the presence of associated radiolabeled or fluorescently labeled proteins. The isolated nuclei exhibited the same specificity for protein uptake as seen previously in vivo, accumulating simian virus 40 wild-type large-T antigen and p53 while excluding a cytoplasmic variant of large-T antigen (d10) and bovine serum albumin. The rapid nuclear accumulation of wild-type large-T antigen was shown to be selective and dependent upon the recognition of a wild-type nuclear location signal, ATP and temperature dependent, and unidirectional. Taken together, the data suggest that in our in vitro system the nuclear translocation of wild-type large-T antigen exhibits some of the characteristics of an active transport process.
机译：描述了一种体外核易位系统，其中将分离的大鼠肝核在含有放射性标记或荧光标记外源蛋白的特定缓冲液中孵育。快速回收，提取并分析核中是否存在相关的放射性标记或荧光标记的蛋白质。分离的核对蛋白质的吸收表现出与先前体内观察到的相同的特异性，积累了猿猴病毒40种野生型大T抗原和p53，同时排除了大T抗原（d10）和牛血清白蛋白的细胞质变体。已证明野生型大T抗原的快速核积累是选择性的，并且取决于对野生型核定位信号，ATP和温度的依赖性以及单向的识别。综上所述，数据表明在我们的体外系统中，野生型大T抗原的核易位表现出主动转运过程的某些特征。

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