A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.

A B Sachs; R W Davis; R D Kornberg

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A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.

机译：酵母多聚（A）结合蛋白的单个域对于RNA结合和细胞生存力是必需的和足够的。

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The poly(A)-binding protein (PAB) gene of Saccharomyces cerevisiae is essential for cell growth. A 66-amino acid polypeptide containing half of a repeated N-terminal domain can replace the entire protein in vivo. Neither an octapeptide sequence conserved among eucaryotic RNA-binding proteins nor the C-terminal domain of PAB is required for function in vivo. A single N-terminal domain is nearly identical to the entire protein in the number of high-affinity sites for poly(A) binding in vitro (one site with an association constant of approximately 2 X 10(7) M-1) and in the size of the binding site (12 A residues). Multiple N-terminal domains afford a mechanism of PAB transfer between poly(A) strands.

机译：酿酒酵母的poly（A）结合蛋白（PAB）基因对于细胞生长至关重要。包含一半重复N端结构域的66个氨基酸的多肽可以在体内替代整个蛋白质。体内功能不需要真核RNA结合蛋白之间保守的八肽序列或PAB的C端结构域。在体外，poly（A）结合的高亲和力位点的数量（一个位点的缔合常数约为2 X 10（7）M-1），单个N末端结构域与整个蛋白质几乎相同。结合位点的大小（12 A残基）。多个N末端结构域提供了多聚（A）链之间PAB转移的机制。

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《Molecular and Cellular Biology》 |1987年第9期|共9页
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A B Sachs; R W Davis; R D Kornberg;
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中图分类细胞生物学;
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1. A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability. [J] . A B Sachs, R W Davis, R D Kornberg Molecular and Cellular Biology . 1987,第9期

机译：酵母多聚（A）结合蛋白的单个域对于RNA结合和细胞生存力是必需的和足够的。
2. DIFFERENTIAL EFFECTS OF AROMATIC AND CHARGED RESIDUE SUBSTITUTIONS IN THE RNA BINDING DOMAINS OF THE YEAST POLY(A) BINDING PROTEIN [J] . Deardorff JA., Sachs AB. Journal of Molecular Biology . 1997,第1期

机译：酵母多聚（A）结合蛋白RNA结合域中芳香和带电残基取代基的差异作用
3. The multiple RNA-binding domains of the mRNA poly(A)-binding protein have different RNA-binding activities. [J] . C G Burd, E L Matunis, G Dreyfuss Molecular and Cellular Biology . 1991,第7期

机译：mRNA poly（A）结合蛋白的多个RNA结合域具有不同的RNA结合活性。
4. UV-induced protein-RNA cross-linking: Identification of RNA-binding domains, peptides, and amino acids [C] . Katharina Kramer, Timo Sachsenberg, Oliver Kohlbacher, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：UV诱导的蛋白质RNA交联：鉴定RNA结合结构域，肽和氨基酸
5. Metal-binding properties of the zinc fingers in metal responsive element-binding transcription factor-1 and design of single-stranded nucleic acid binding proteins using nucleocapsid-like CCHC zinc-binding domains. [D] . Guerrerio, Anthony Lawrence, Jr. 2004

机译：锌指在金属反应元件结合转录因子-1中的金属结合特性，以及使用核衣壳样CCHC锌结合结构域设计单链核酸结合蛋白。
6. A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability. [O] . A B Sachs, R W Davis, R D Kornberg 1987

机译：酵母多聚（A）结合蛋白的单个域对于RNA结合和细胞生存力是必需的和充分的。
7. A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability. [O] . Sachs, A B, Davis, R W, Kornberg, R D 1987

机译：酵母多聚（A）结合蛋白的单个域对于RNA结合和细胞生存力是必需的和充分的。

A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.

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