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首页> 外文期刊>Molecular and Cellular Biology >The excised leader of human cytochrome c oxidase subunit I mRNA which contains the origin of mitochondrial DNA light-strand synthesis accumulates in mitochondria and is polyadenylated.
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The excised leader of human cytochrome c oxidase subunit I mRNA which contains the origin of mitochondrial DNA light-strand synthesis accumulates in mitochondria and is polyadenylated.

机译:包含线粒体DNA轻链合成起点的人类细胞色素c氧化酶亚基I mRNA的前导片段堆积在线粒体中,并被聚腺苷酸化。

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摘要

We identified a polyadenylated RNA species which contains the origin of human mitochondrial DNA light-strand synthesis and the surrounding complementary sequences of the four light-strand-encoded tRNAs. This RNA (RNA 9L) is probably derived from the leader portion of RNA 6 which is excised during the formation of the mature cytochrome c oxidase subunit mRNA (RNA 9). The high degree of secondary structure of this RNA is presumably responsible for its anomalous electrophoretic behavior in denaturing polyacrylamide gels.
机译:我们确定了一个聚腺苷酸化的RNA物种,其中包含人类线粒体DNA轻链合成的起源和周围的四个轻链编码tRNA的互补序列。该RNA(RNA 9L)可能源自RNA 6的前导部分,该部分在成熟的细胞色素C氧化酶亚基mRNA(RNA 9)形成过程中被切除。这种RNA的高度二级结构可能是变性聚丙烯酰胺凝胶中其异常电泳行为的原因。

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