Identification of the upstream activating sequence of MAL and the binding sites for the MAL63 activator of Saccharomyces cerevisiae.

B F Ni; R B Needleman

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Identification of the upstream activating sequence of MAL and the binding sites for the MAL63 activator of Saccharomyces cerevisiae.

机译：鉴定啤酒酵母的上游激活序列和MAL63激活子的结合位点。

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Maltose fermentation in Saccharomyces species requires the presence of at least one of five unlinked MAL loci: MAL1, MAL2, MAL3, MAL4, and MAL6. Each of these loci consists of a complex of genes involved in maltose metabolism; the complex includes maltase, a maltose permease, and an activator of these genes. At the MAL6 locus, the activator is encoded by the MAL63 gene. While the MAL6 locus has been the subject of numerous studies, the binding sites of the MAL63 activator have not been determined. In this study, we used Escherichia coli extracts containing the MAL63 protein to define the binding sites of the MAL63 protein in the divergently transcribed MAL61-62 promotor. When a DNA fragment containing these sites was placed upstream of a CYC1-lacZ gene, maltose induced beta-galactosidase. These sites therefore constitute an upstream activating sequence for the MAL genes.

机译：酵母菌种中的麦芽糖发酵需要存在五个未连接的MAL基因座中的至少一个：MAL1，MAL2，MAL3，MAL4和MAL6。这些基因座均由与麦芽糖代谢有关的基因复合体组成。该复合物包括麦芽糖酶，麦芽糖通透酶和这些基因的激活剂。在MAL6位点，激活物由MAL63基因编码。尽管MAL6基因座已成为众多研究的主题，但尚未确定MAL63激活剂的结合位点。在这项研究中，我们使用含有MAL63蛋白的大肠杆菌提取物来定义在不同转录的MAL61-62启动子中MAL63蛋白的结合位点。当将包含这些位点的DNA片段置于CYC1-lacZ基因的上游时，麦芽糖诱导了β-半乳糖苷酶。因此，这些位点构成了MAL基因的上游激活序列。

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《Molecular and Cellular Biology》 |1990年第7期|共4页
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B F Ni; R B Needleman;
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中图分类细胞生物学;
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1. Characterization of the DNA-binding activity of GCR1: in vivo evidence for two GCR1-binding sites in the upstream activating sequence of TPI of Saccharomyces cerevisiae. [J] . M A Huie, E W Scott, C M Drazinic, Molecular and Cellular Biology . 1992,第6期

机译：GCR1的DNA结合活性的表征：体内证据表明酿酒酵母TPI的上游激活序列中有两个GCR1结合位点。
2. Identification of an upstream activating sequence and an upstream repressible sequence of the pyruvate kinase gene of the yeast Saccharomyces cerevisiae. [J] . M Nishizawa, R Araki, Y Teranishi Molecular and Cellular Biology . 1989,第2期

机译：鉴定酿酒酵母酵母丙酮酸激酶基因的上游激活序列和上游可抑制序列。
3. Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae. [J] . A R Buchman, W J Kimmerly, J Rine, Molecular and Cellular Biology . 1988,第1期

机译：酿酒酵母中的两个DNA结合因子识别沉默子处的特定序列，上游激活序列，自主复制序列和端粒。
4. Phosphoenolpyruvate carboxylase (PEPC): mutational analysis of a flexible loop and a putative binding site for an allosteric activator, glucose 6-phosphate (G6P) [C] . A Terada, M Kotera, K Tsumura, International Congress on Photosynthesis . 2001

机译：磷酸丙烯酸盐羧基（Pepc）：柔性环的突变分析和血糖活化剂的推定结合位点，葡萄糖6-磷酸（G6P）
5. Upstream activation sequence function in Saccharomyces cerevisiae: The potential for long distance transcriptional activation and the role of Sin4. [D] . Dobi, Krista Carol. 2007

机译：酿酒酵母中的上游激活序列功能：长距离转录激活的潜力和Sin4的作用。
6. Identification of the upstream activating sequence of MAL and the binding sites for the MAL63 activator of Saccharomyces cerevisiae. [O] . B F Ni, R B Needleman 1990

机译：鉴定啤酒酵母的MAL的上游激活序列和MAL63激活子的结合位点。
7. Identification of the upstream activating sequence of MAL and the binding sites for the MAL63 activator of Saccharomyces cerevisiae. [O] . Ni, B F, Needleman, R B 1990

机译：鉴定啤酒酵母的MAL的上游激活序列和MAL63激活子的结合位点。

Identification of the upstream activating sequence of MAL and the binding sites for the MAL63 activator of Saccharomyces cerevisiae.

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