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首页> 外文期刊>Molecular and Cellular Biology >Regulated expression of globin chains and the erythroid transcription factor GATA-1 during erythropoiesis in the developing mouse.
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Regulated expression of globin chains and the erythroid transcription factor GATA-1 during erythropoiesis in the developing mouse.

机译：在发育中的小鼠的红细胞生成过程中，球蛋白链和红系转录因子GATA-1的表达调控。

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Erythropoiesis in vertebrates is characterized by sequential changes in erythropoietic site, erythroblast morphology, and hemoglobin synthesis. We have examined the expression of globin chains and the major erythroid transcription factor GATA-1 (previously known as GF-1/NF-E1/Eryf 1) from days 7.5 to 17.5 of mouse development. mRNAs for embryonic (epsilon y2, beta H1, and zeta) and adult (alpha and beta) globin chains were quantitated by RNase protection assays. Switching of globins within the alpha-globin cluster (alpha and zeta) was not strictly coordinated with that within the beta-globin cluster (epsilon y2, beta H1, and beta). Regulation of globin switches during development was primarily transcriptional. Of particular note, we found two developmental switches (beta H1 to epsilon y2 and epsilon y2 to beta) in the mouse, more analogous than previously thought to shifts found in human development. The erythroid transcription factor GATA-1, believed to be a principal regulator of genes expressed in erythroid cells, first appeared in the embryo in yolk sac at the time of blood island formation and remained at a low level during embryonic erythropoiesis (8 to 11 days) relative to that found later in fetal liver (12 to 15 days). The rise in GATA-1 mRNA in fetal liver paralleled and preceded the rapid accumulation of adult beta-globin RNA. RNase protection assays and a GATA-1-specific peptide antiserum were used to establish that a single GATA-1 polypeptide is expressed throughout mouse development. Overall, these findings suggest that the levels of this erythroid transcription factor during development may contribute to the differential gene activation characteristic of definitive versus primitive erythropoiesis.
机译：脊椎动物的红细胞生成的特征是红细胞生成部位，红细胞形态和血红蛋白合成的顺序变化。我们已经从小鼠发育的7.5天到17.5天检查了珠蛋白链和主要的类红细胞转录因子GATA-1（以前称为GF-1 / NF-E1 / Eryf 1）的表达。胚胎（ε2，βH1和zeta）和成年（α和β）球蛋白链的mRNA通过RNase保护测定进行定量。 α-球蛋白簇（α和zeta）中的球蛋白转换与β-球蛋白簇（εy2，βH1和beta）中的球蛋白转换并不严格协调。在发育过程中对球蛋白开关的调节主要是转录。特别值得注意的是，我们在小鼠中发现了两个发育开关（从βH1到ε2和从y2到β），比以前认为的人类发展变化更相似。红血球转录因子GATA-1，被认为是红血球细胞中表达的基因的主要调节因子，在血岛形成时首先出现在卵黄囊的胚胎中，并在胚胎红细胞生成过程中（8至11天）保持较低水平）（相对于后来在胎儿肝脏（12至15天）中发现的）。胎儿肝脏中GATA-1 mRNA的升高与成年β-珠蛋白RNA的快速积累平行且先于后者。 RNase保护测定和GATA-1特异性肽抗血清用于确定在整个小鼠发育过程中均表达单个GATA-1多肽。总体而言，这些发现表明，发育过程中该类红细胞转录因子的水平可能有助于确定性促红细胞生成与原始促红细胞生成的差异基因激活特征。

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