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首页> 外文期刊>Molecular and Cellular Biology >Transcripts from amplified sequences of an inherited homogeneously staining region in chromosome 1 of the house mouse (Mus musculus).
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Transcripts from amplified sequences of an inherited homogeneously staining region in chromosome 1 of the house mouse (Mus musculus).

机译：来自家鼠（小家鼠）1号染色体上一个均质染色区遗传序列的扩增序列的转录本。

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摘要

Several populations of the house mouse, Mus musculus, are polymorphic for the presence or absence of an inherited homogeneously staining region (HSR) in chromosome 1. The HSR consists of highly amplified DNA sequences, present in low copy numbers in the HSR- genome. A cloned HSR-derived genomic sequence detected transcripts of about 1.3 and 4.5 kb on blots of poly(A)+ RNA from liver of HSR+ mice but not from that of HSR- mice. A cDNA library was established from RNA of HSR+ mice and screened with the HSR-derived genomic clone. Positive clones were isolated and shown to be complementary to the 1.3-kb RNA species and to amplified DNA sequences in the HSR+ genome. The combined sequence of four overlapping cloned cDNAs is 959 nucleotides long and includes an open reading frame encoding a putative protein of 208 amino acids. The pertinent gene is unidentified. No homologous sequence is stored in the EMBL data base. A stretch of 109 nucleotides at the 3' end of the 1.3-kb RNA homology region in the same genomic fragment, as indicated by hybridization data and sequence motifs resembling promoter elements. Thus, our data suggest that at least two genes or gene families are encoded in the HSR.
机译：由于在1号染色体上是否存在遗传的均匀染色区（HSR），家鼠的一些种群具有多态性。HSR由高度扩增的DNA序列组成，在HSR基因组中以低拷贝数存在。克隆的HSR衍生基因组序列在来自HSR +小鼠肝脏而不是HSR-小鼠肝脏的poly（A）+ RNA印迹上检测到约1.3和4.5 kb的转录本。从HSR +小鼠的RNA建立cDNA文库，并用HSR衍生的基因组克隆进行筛选。分离出阳性克隆，显示出与1.3-kb RNA物种和HSR +基因组中扩增的DNA序列互补。四个重叠克隆的cDNA的组合序列长959个核苷酸，并包括一个编码208个氨基酸的推定蛋白的开放阅读框。相关基因尚未鉴定。 EMBL数据库中没有存储任何同源序列。杂交数据和类似于启动子元件的序列基序表明，同一基因组片段中1.3-kb RNA同源区域的3'端有109个核苷酸的片段。因此，我们的数据表明在HSR中至少编码了两个基因或基因家族。

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