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首页> 外文期刊>Molecular and Cellular Biology >Cloning of DNA corresponding to rare transcripts of rat brain: evidence of transcriptional and post-transcriptional control and of the existence of nonpolyadenylated transcripts.
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Cloning of DNA corresponding to rare transcripts of rat brain: evidence of transcriptional and post-transcriptional control and of the existence of nonpolyadenylated transcripts.

机译：克隆与大鼠脑稀有转录物相对应的DNA：转录和转录后控制以及非聚腺苷酸化转录物存在的证据。

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To examine the expression of genes encoding rare transcripts in the rat brain, we have characterized genomic DNA clones corresponding to this class. In brain cells, as in all cell types, rare transcripts constitute the majority of different sequences transcribed. Moreover, when compared with other tissues or cultured cells, brain tissue may be expected to have an even larger set of rare transcripts, some of which could be restricted to subpopulations of neural cells. We have identified seven clones whose transcripts are nonabundant, averaging less than three copies per cell. Clone rg13 (rat genomic 13) RNA was detected only in the brain, whereas RNA of a second clone, rg40, was also detected in the brain and in a melanoma. Transcripts of rg13 were found in cerebellum, cerebral cortex, and regions underlying the cortex, whereas rg40 transcripts were not detected in the cerebellum. Transcripts of both rg13 and rg40 were found in pelleted polysomal RNA. RNA of another clone, rg34, was found in the brain, liver, and kidney but was found in pelleted polysomal RNA only in the brain, suggesting that its expression may be post-transcriptionally controlled. The remaining four clones represent rare transcripts that are common to the brain, liver, and kidney; rg18 RNA is restricted to the nucleus, whereas rg3, rg26, and rg36 transcripts are found in the cytoplasm of all three tissues. Transcripts of the brain-specific clone, rg13, and the commonly expressed clone, rg3, are nonpolyadenylated, presumably belonging to the high-complexity, nonpolyadenylated class of transcripts in the mammalian brain.
机译：为了检查在大鼠脑中编码稀有转录本的基因的表达，我们鉴定了与此类相对应的基因组DNA克隆。与所有细胞类型一样，在脑细胞中，稀有的转录本构成了转录的大多数不同序列。此外，当与其他组织或培养的细胞相比时，可以预期脑组织具有甚至更多的稀有转录本，其中一些可能限于神经细胞亚群。我们已经鉴定出七个转录本不丰富的克隆，每个细胞平均少于三个拷贝。仅在大脑中检测到了克隆rg13（大鼠基因组13）RNA，而在大脑和黑色素瘤中也检测到了第二个克隆rg40的RNA。在小脑，大脑皮层和皮层下的区域中发现了rg13的转录本，而在小脑中未检测到rg40的转录本。在沉淀的多体RNA中发现了rg13和rg40的转录本。在大脑，肝脏和肾脏中发现了另一个克隆rg34的RNA，但仅在大脑中在沉淀的多体RNA中发现了rg34，这表明其表达可能受转录后控制。其余四个克隆代表大脑，肝脏和肾脏共有的稀有转录本。 rg18 RNA仅限于细胞核，而在所有三种组织的细胞质中均发现rg3，rg26和rg36转录本。脑特异性克隆rg13和通常表达的克隆rg3的转录本是非聚腺苷酸化的，大概属于哺乳动物脑中高复杂性，非聚腺苷酸化的转录本类别。

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