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首页> 外文期刊>Molecular and Cellular Biology >Antipeptide antiserum identifies a widely distributed cellular tyrosine kinase related to but distinct from the c-fps/fes-encoded protein.
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Antipeptide antiserum identifies a widely distributed cellular tyrosine kinase related to but distinct from the c-fps/fes-encoded protein.

机译：抗肽抗血清可识别与c-fps / fes编码蛋白相关但又不同的细胞酪氨酸激酶。

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摘要

We raised antibodies directed against a synthetic peptide representing an amino acid sequence of the conserved kinase domain of the transforming protein of Fujinami sarcoma virus (FSV) (P140). The antiserum obtained specifically recognized FSV-P140 and its cellular homolog and in addition, it recognized a new cellular protein of 94,000 daltons (NCP94) in avian and mammalian cells. NCP94 was found to be associated with a cyclic nucleotide-independent protein kinase activity that was specific for tyrosine residues. Although NCP94 and FSV-P140 share antigenic determinants, NCP94 is not a cellular homolog of FSV-P140: NCP94 and the previously identified c-fps/fes product were different in their tryptic fingerprints and in their tissue specificities. Thus, the function of NCP94 in normal cells is probably different than that of the c-fps/fes product. NCP94 was expressed in every tissue and cell line that was examined. In chickens, NCP94 levels were highest during embryonic development and NCP94 expression was high in gizzard, brain, and spleen throughout embryonic and adult life. The universal expression of NCP94 suggests that this protein may be involved in an essential function of normal cells. NCP94 may be a new cellular tyrosine kinase of the src gene family.
机译：我们提出了针对合成肽的抗体，该肽代表藤蔓肉瘤病毒（FSV）（P140）转化蛋白保守激酶结构域的氨基酸序列。获得的抗血清可以特异性识别FSV-P140及其细胞同源物，此外，它还可以识别禽类和哺乳动物细胞中94,000道尔顿的新细胞蛋白（NCP94）。发现NCP94与酪氨酸残基特异的环状核苷酸无关蛋白激酶活性有关。尽管NCP94和FSV-P140共享抗原决定簇，但NCP94不是FSV-P140的细胞同源物：NCP94和先前鉴定的c-fps / fes产物的胰蛋白酶指纹和组织特异性不同。因此，NCP94在正常细胞中的功能可能与c-fps / fes乘积的功能不同。 NCP94在检查的每个组织和细胞系中都有表达。在鸡中，整个胚胎和成年期中，NCP94的水平在胚胎发育过程中最高，而g，大脑和脾脏中的NCP94的表达水平很高。 NCP94的普遍表达表明该蛋白可能参与正常细胞的基本功能。 NCP94可能是src基因家族的一种新型细胞酪氨酸激酶。

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