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首页> 外文期刊>Molecular and Cellular Biology >Effects of progressive depletion of TCM1 or CYH2 mRNA on Saccharomyces cerevisiae ribosomal protein accumulation.
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Effects of progressive depletion of TCM1 or CYH2 mRNA on Saccharomyces cerevisiae ribosomal protein accumulation.

机译：TCM1或CYH2 mRNA的逐步耗竭对酿酒酵母核糖体蛋白积累的影响。

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摘要

When present in excess, the mRNAs for Saccharomyces cerevisiae ribosomal proteins L3 and L29 are translated less efficiently, so that synthesis of these proteins remains commensurate with that of other ribosomal proteins (N.J. Pearson, H.M. Fried, and J.R. Warner, Cell 29:347-355, 1982; J.R. Warner, G. Mitra, W.F. Schwindinger, M. Studeny, and H.M. Fried, Mol. Cell. Biol. 5:1512-1521, 1985). We used a yeast strain with a conditionally transcribed derivative of the L3 gene to deplete cells progressively of L3 mRNA. In this case translation of L3 mRNA did not become more efficient so that L3 was not maintained at a normal level. Even when there was an initial excess of L3 mRNA, interruption of its further transcription produced an immediate drop in L3 synthesis, suggesting that the translational efficiency of preexisting mRNA cannot be altered. Lack of L3 synthesis afforded an opportunity to examine coordinate accumulation of other ribosomal proteins. Without L3, apparent synthesis of several 60S subunit proteins diminished, and 60S subunits did not assemble. A similar phenomenon occurred when, in a second strain, synthesis of ribosomal protein L29 was prevented. Loss of 60S subunit assembly was accompanied by a destabilization of some 60S ribosomal protein mRNAs. These data suggest that synthesis of some S. cerevisiae ribosomal proteins may be regulated posttranscriptionally as a function of the extent to which they are assembled.
机译：当过量存在时，酿酒酵母核糖体蛋白L3和L29的mRNA转换效率较低，因此这些蛋白的合成与其他核糖体蛋白的合成保持相对应（NJ Pearson，HM Fried和JR Warner，Cell 29：347- ，第355页，1982; JR Warner，G.Mitra，WF Schwindinger，M.Studeny和HM Fried，分子细胞生物学5：1512-1521，1985）。我们使用带有L3基因条件转录衍生物的酵母菌株逐步消耗L3 mRNA的细胞。在这种情况下，L3 mRNA的翻译没有变得更有效，因此L3不能维持在正常水平。即使当最初存在过量的L3 mRNA时，其进一步转录的中断也会导致L3合成立即下降，这表明先前存在的mRNA的翻译效率无法改变。 L3合成的缺乏为检查其他核糖体蛋白的坐标积累提供了机会。没有L3，几个60S亚基蛋白质的表观合成就减少了，并且60S亚基没有组装。当在第二个菌株中阻止了核糖体蛋白L29的合成时，发生了类似的现象。 60S亚基装配的丧失伴随着一些60S核糖体蛋白mRNA的失稳。这些数据表明，某些酿酒酵母核糖体蛋白的合成可能根据其组装程度而在转录后受到调控。

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